- Clone
- A20078A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- S-Endo 1 antigen, MUC18, MCAM, Mel-CAM, A32 antigen
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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112002 | 100 µg | 88€ |
CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30% of neutrophils and 60% of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFN-γ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant protein (val 24-val 563 extracellular domain)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2927859 (BioLegend Cat. No. 112002)
Antigen Details
- Structure
- CD146 is a transmembrane glycoprotein and belongs to the Ig superfamily of cell adhesion molecules.
- Distribution
-
CD146 is strongly expressed by murine vascular endothelial cells, a subset of NK1.1+ cells, and neutrophils.
- Function
- CD146 has been reported to be functionally relevant for endothelial cell adhesion, angiogenesis, and NK cell function.
- Ligand/Receptor
- Unidentified ligand
- Cell Type
- Endothelial cells, Epithelial cells, Mesenchymal cells, Mesenchymal Stem Cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Gene ID
- 84004 View all products for this Gene ID
- UniProt
- View information about CD146 on UniProt.org
Related FAQs
Other Formats
View All CD146 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD146 | A20078A | FC,IHC-F |
Alexa Fluor® 488 anti-mouse CD146 | A20078A | FC,IHC-F |
Alexa Fluor® 594 anti-mouse CD146 | A20078A | FC |
Alexa Fluor® 647 anti-mouse CD146 | A20078A | FC,IHC-F |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD146
bEnd.3 cells (mouse endothelial cells) were stained with pur... C57BL/6 mouse frozen kidney section was fixed with 4% parafo... -
Alexa Fluor® 488 anti-mouse CD146
C57BL/6 mouse splenocytes were stained with anti-mouse NK-1.... IHC staining of Alexa Fluor® 488 anti-mouse CD146 (clone... -
Alexa Fluor® 594 anti-mouse CD146
C57BL/6 mouse splenocytes were stained with anti-mouse NK-1.... -
Alexa Fluor® 647 anti-mouse CD146
C57BL/6 mouse splenocytes were stained with anti-mouse NK-1.... IHC staining of anti-mouse CD146 (clone A20078A) Alexa Fluor...
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