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Purified anti-mouse CD197 (CCR7) Antibody

Pricing & Availability
Clone
4B12 (See other available formats)
Regulatory Status
RUO
Other Names
C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled receptor 1, EBI-1, CD197
Isotype
Rat IgG2a, κ
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Product Citations
publications
4B12_pure_CD197_Antibody_1_030818
C57BL/6 splenocytes stained with CD197 (clone 4B12) purified and anti-rat IgG2a, κ (clone RTK2758) FITC and CD3ε (145-2C11) APC
  • 4B12_pure_CD197_Antibody_1_030818
    C57BL/6 splenocytes stained with CD197 (clone 4B12) purified and anti-rat IgG2a, κ (clone RTK2758) FITC and CD3ε (145-2C11) APC
  • 4B12_pure_CD197_Antibody_2_030518
    Peyer’s patches of a CX3CR1-GFP -/+ mouse were fixed and cryosectioned in OCT. Following blocking and permeabilization of tissues slices, they were stained overnight at 4°C with the primary antibodies Rt anti-CCR7/CD197 (4B12), Hm anti-CD11c (N418), Chk anti-GFP followed by 1h at RT with the secondary antibodies Dk anti-rat Cy3 (pink), Gt anti-hamster Alexa Fluor® 594 (red), and Dk anti-chicken Alexa Fluor® 488 (green/yellow). Following additional blocking, sections were then stained with anti-CD4 (RM4-5) eFluor 450 (cyan) for one hour, washed in PBS and mounted in Prolong Gold™. Merged image is on the left and single color image of anti-CCR7/CD197 is on the right. Image Courtesy of Hugues Lelouard, Centre d’immunologie de Marseille-Luminy.
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120101 50 µg 104€
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Description

CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta) and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and chemotaxis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CCR7 transfected RBL-2H3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Customer Reported
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 4B12 antibody does not inhibit binding of ligand to receptor. Additional reported applications (for the relevant formats) include: immunoprecipitation. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302) is recommended prior to immunofluorescent staining.

Staining with clone 4B12 is recommended at 37°C (see supplemental data of PE staining at differing temperatures).

Application References
  1. Ohl L, et al. 2004. Immunity 21:279.
  2. Ritter U, et al. 2004. J. Leukocyte Biol. 76:472.
  3. Lan YY, et al. 2005. Am. J. Transplant. 5:2649. (FC)
  4. Lee JH, et al. 2007. J. Immunol. 178:301. (FC) PubMed
  5. Kurooka M and Kaneda Y. 2007. Cancer Res. 67:227. (FC) PubMed
  6. Thompson BD. 2007. J. Biol. Chem. 282:9547. (FC)
  7. Sakai N, et al. 2006. P. Natl. Acad. Sci. USA 103:14098. (FC)
  8. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC)
  9. Hwang IY, et al. 2007. J. Immunol. 179:439. (FC) PubMed
  10. Kang SG, et al. 2007. J. Immunol. 179:3724.
  11. Mao A et al. 2005. J. Immunol. 175:5146. PubMed
  12. Allende ML, et al. 2008. FASEB J. 22:307. PubMed
  13. Kang SG, et al. 2007. J. Immunol. 179:3724. PubMed
  14. Chen H, et al. 2005. J. Immunol. 175:591. PubMed
  15. Florido M, et al. 2009. Immunobiology. 214:643. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
Product Citations
  1. Yu H, et al. 2020. Front Immunol. 11:1773. PubMed
  2. Kang S, et al. 2007. J Immunol. 179:3724. PubMed
  3. Ishizaki H, et al. 2006. J Immunol. 177:8512. PubMed
  4. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  5. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  6. Lee J, et al. 2007. J Immunol. 178:301. PubMed
  7. Thompson B, et al. 2007. J Biol Chem. 282:9547. PubMed
  8. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  9. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  10. Hwang I, Kehrl C 2004. J Immunol. 179:439. PubMed
  11. Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed
  12. Ghilas S, et al. 2021. iScience. 24:103059. PubMed
  13. Barvalia M, et al. 2022. Methods Mol Biol. 2508:147. PubMed
  14. He LD, et al. 2021. EMBO Rep. 22:e52196. PubMed
  15. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  16. Mao A, et al. 2005. J Immunol. 175:5146. PubMed
  17. Iida Y, et al. 2020. J Immunother Cancer. 8:. PubMed
  18. Platteel ACM, et al. 2018. Front Immunol. 1.544444444. PubMed
  19. Kaneda M 2007. Cancer Res. 67:227. PubMed
  20. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
RRID
AB_389229 (BioLegend Cat. No. 120101)

Antigen Details

Structure
Rhodopsin-like GPCR superfamily, G-protein coupled receptor 1 family, integral membrane protein, predicted molecular weight 43 kD
Distribution

Hematopoietic stem cells, T subsets, mature dendritic cells

Function
Homozygous mutation. Receptor for MIP-3 beta and SLC chemokines. Probable mediator of EBV effects on B lymphocytes. Plays a role in T cell adhesion at intestinal sites; may also play a role in the homeostatic recirculation of memory T cells and chemotaxis.
Ligand/Receptor
MIP3 beta, SLC (6CKine, Exodus-2, TCA-4)
Cell Type
Hematopoietic stem and progenitors, T cells, Dendritic cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Schweickart VL, et al. 1994. Genomics 23:643.
2. Yoshida R, et al. 1998. J. Biol. Chem. 273:7118.
3. Campbell JJ, et al. 1998. J. Cell Biol. 141:1053.
4. Willimann K, et al. 1998. Eur. J. Immunol. 28:2025.

Gene ID
12775 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 4    Revision Date: 03/05/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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