- Clone
- SER211 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Homeobox Transcription Factor Nanog, Nanog Homeobox, Homeobox Protein NANOG, Homeobox Transcription Factor Nanog-Delta 48, ENK, ecat4
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
678102 | 100 µg | 231€ |
Nanog is a transcription factor that plays a key role in the maintenance of embryonic stem cells pluripotency and self-renewal in embryonic stem cells. Nanog expression is regulated by other transcription factors such as Sox2 and Oct-4. Nanog also plays a role in epithelial malignancy. Nanog is enriched in cancer stem cells and the ablation of Nanog is sufficient to reduce the cancer stem cell pool.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Nanog.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
A non-specific band of roughly 60 kD was observed in some lysates during product development testing. - RRID
-
AB_2565914 (BioLegend Cat. No. 678102)
Antigen Details
- Structure
- 305 amino acids wtih a predicted molecular weight of approximately 35 kD.
- Distribution
-
Nuclear and cytoplasmic. Expressed by embryonic stem (ES) cells or cancer stem cells. Expression decreases with ES differentiation.
- Function
- Transcription regulator that plays key roles in the maintenance of pluripotency and self-renewal in embryonic stem cells.
- Interaction
- Interacts with SMAD1, SALL4, and ZNF281/ZFP281.
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Signal Transduction, Stem Cells, Transcription Factors
- Antigen References
-
1. Hwang CC, et al. 2014. PLoS One 9:e105676.
2. Torres-Padilla ME, et al. 2014. Development 141:2173.
3. Xie X, et al. 2014. Int. J. Cancer 12:2741.
4. Wang ML, et al. 2013. Onco. Targets Ther. 6:1207.
5. Kim J, et al. 2008. Cell 132:1049.
6. Takahashi K, et al. 2007. Nat. Protoc. 2:3081.
7. Yu J, et al. 2007. Science 318:1917. - Gene ID
- 71950 View all products for this Gene ID
- UniProt
- View information about Nanog on UniProt.org
Related FAQs
Other Formats
View All Nanog Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Nanog | SER211 | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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