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Purified anti-NLRP12 Antibody

Pricing & Availability
Clone
3D11A53 (See other available formats)
Regulatory Status
RUO
Other Names
NACHT, LRR and PYD domains-containing protein 12, Monarch-1, PYRIN-containing APAF1-like protein 7 (PYPAF7), Regulated by nitric oxide (RNO)
Isotype
Mouse IgG2b, κ
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Product Citations
publications
3D11A53_Purified_NLRP12_Antibody_WB_022715
Total cell lysate from HL-60 cells (lane 1) or DMSO treated HL-60 cells (1.25% DMSO treated for five days, lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NLRP12 (clone 3D11A53) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 3D11A53_Purified_NLRP12_Antibody_WB_022715
    Total cell lysate from HL-60 cells (lane 1) or DMSO treated HL-60 cells (1.25% DMSO treated for five days, lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-NLRP12 (clone 3D11A53) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
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675202 100 µg 249€
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Description

NLRP12, a member of the Nod-like receptor (NLR) family of proteins, was originally suggested to act as an inflammasome forming component and a modulator of NF-κB signaling. Recent studies revealed that NLRP12 plays anti-inflammatory roles, as NLRP12-deficient mice are more susceptible to chemically induced colitis, and show a more severe infiltration of granulocytes and T cells. The colitis induced in NLRP12-/- mice is associated with increased production of proinflammatory cytokines and activation of both NF-κB and ERK signaling pathways. NLRP12 was reported to attenuate canonical NF-κB signaling by inhibiting IRAK-1 phosphorylation. NLRP12 also down-regulates non-canonical NF-κB signaling through interaction with TRAF3 and the NF-κB inducing kinase NIK.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human NLRP12 recombinant protein (93-1061 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2565493 (BioLegend Cat. No. 675202)

Antigen Details

Structure
1061 amino acids with a predicted molecular weight of 120 kD. Contains a DAPIN domain, a NACHT-associated domain, and eight leucine-rich repeats (LRR).
Distribution
Cytoplasm.
Function
NLRP12 negatively regulatres inflammation through attenuating both canonical and non-canonical NF-κB signaling.
Interaction
Interacts with ASC and FAF1 through its DAPIN domain.
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Allen IC. 2014. Front Immunol. 5:169.
2. Zaki MH, et al. 2014. Proc. Natl. Acad. Sci. USA 111:385.
3. Chen GY. 2014. Eur. J. Immunol. 44:321.
4. Vladimer GI. 2013. Curr. Opin. Microbiol. 16:23.
5. Allen IC, et al. 2012. Immunity 36:742.
6. Vladimer GI, et al. 2012. Immunity 37:96.

Gene ID
91662 View all products for this Gene ID
UniProt
View information about NLRP12 on UniProt.org
Go To Top Version: 1    Revision Date: 02/27/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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