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Purified anti-NLRP2 Antibody

Pricing & Availability
Clone
8F10B51 (See other available formats)
Regulatory Status
RUO
Other Names
NACHT, LRR and PYD domains-containing protein 2, Nucleotide-binding site protein 1 (NBS-1), NBS1, PYRIN domain and NACHT domain-containing protein 1 (PAN1), PYRIN-containing APAF1-like protein 2 (PYPAF2)
Isotype
Mouse IgG1, κ
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Product Citations
publications
8F10B51_PURE_NLRP2_Antibody_1_WB_051016
Western blot analysis of A431 (lane 1), Jurkat (lane 2), and NIH3T3 (lane 3) using anti-NLRP2 (clone 8F10B51) antibody followed by HRP goat anti-mouse IgG antibody. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
  • 8F10B51_PURE_NLRP2_Antibody_1_WB_051016
    Western blot analysis of A431 (lane 1), Jurkat (lane 2), and NIH3T3 (lane 3) using anti-NLRP2 (clone 8F10B51) antibody followed by HRP goat anti-mouse IgG antibody. Purified anti-β-actin (clone Poly6221) antibody was used as a loading control.
  • 8F10B51_PURE_NLRP2_Antibody_2_ICC_011421
    A431 cells were stained with purified anti-NLRP2 (clone 8F10B51) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG antibody (green) and DAPI (blue).
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673902 100 µg 212€
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Description

NLRP2, also known as NALP2 or PAN1, is a member of the pyrin domain-containing NLR protein (NLRP) family. The NLR proteins interact with ASC via the conserved pyrin domain, which results in the activation of caspase-1 and maturation of the pro-inflammatory cytokines IL-1β and IL-18. NLRP2 is also involved in the formation of the inflammasome and caspase-1 activation. NLRP2 negatively regulates NFκB activation, which is induced by TNF-α, through inhibiting NFKBIA degradation. In addition, NLRP2 is one of the maternal effect genes and plays a crucial role in embryonic development. In mouse embryos, knockdown or mutation of NLRP2 leads to developmental defections. Truncation of the NLRP2 gene is reported to associate with an overgrowth discorder called Beckwith-Wiedemann Syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial NLRP2 recombinant protein (60-192 aa) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
Upon receipt, store undiluted at 4°C
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564486 (BioLegend Cat. No. 673902)

Antigen Details

Structure
1062 amino acids with a predicted molecular weight of 120.5 kD; contains a DAPIN domain, NACT domain, and eight LRR repeats.
Distribution

Cytoplasm

Function
NLRP2 is a component of inflammasome that is involved in the activation of caspase-1 and the inhibition of NK&κB signaling.
Interaction
NLRP2 interacts wtih IKBKB, IKBKG, CHUK, and MEFV.
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Peng H, et al. 2012. PLoS One 7:e30344.
2. Lupfer C, et al. 2013. Front Immunol. 4:285.
3. Minkiewicz J, et al. 2013. Glia. 61:1113.
4. Huang JY, et al. 2013. Hum. Reprod. 28:1127.
5. Fontalba A, et al. 2007. J. Immunol. 179:8519.
6. Bruey JM, et al. 2004. J. Biol. Chem. 50:51897.

Gene ID
55655 View all products for this Gene ID
UniProt
View information about NLRP2 on UniProt.org
Go To Top Version: 3    Revision Date: 01/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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