- Clone
- RL2 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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677902 | 100 µg | 278€ |
The monoclonal antibody RL2 reacts specifically with beta-O-linked N-acetylglucosamine (O-GlcNAc). It recognizes o-GlcNAc in beta O-glycosidic linkage to both serine and threonine. However, it exhibits no cross reactivity with alpha-linked Ser-O-linked N-acetylgalactosamine or N-linked oligosaccharides on ovalbumin and immunoglobulin. This antibody readily detects in vivo changes in O-GlcNAc modification1.
O-GlcNAc is one of the most abundant posttranslational modifications within the nucleocytoplasmic compartments of all metazoans2. O-GlcNAcylation is similar to protein phosphorylation. Research on O-GlcNAc is particularly relevant to chronic human diseases such as diabetes, cardiovascualr disease, neurodegenerative disorders, and cancer3.
Like phosphorylation, the specific functions of O-GlcNAc depend on the protein and sites to which the glycan is attached2. One of the major functions of O-GlcNAc is to prevent O-phosphorylation and to modulate signaling and transcription in response to cellular nutrients or stress4.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- Other species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- O-GlcNAc in beta-O-glycosidic linkage to both serine and threonine.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IHC, IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
-
- Snow CM, et al. 1987. J. Cell Biol. 104:1143.
- Shin SH, et al. 2011. Amino Acids 40:885-93.
- Mi W, et al. 2011. Biochim. Biophys. Acta. 4:514.
- Hoedt E, et al. 2010. J. Biol. Chem. 285:19205.
- Product Citations
-
- RRID
-
AB_2565912 (BioLegend Cat. No. 677902)
Antigen Details
- Structure
- O-GlcNAc modified protein.
- Distribution
-
Nucleus and cytoplasm.
- Function
- To prevent O-phosphorylation and to modulate signaling and transcription in response to cellular nutrients or stress.
- Biology Area
- Cell Biology, Signal Transduction, Transcription Factors
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Comer FI, et al. 2001. Anal Biochem. 293:169.
2. Hart, GW and Akimoto, Y. (2009) Essentials of Glycobiology, 2nd edition. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
3. de Queiroz RM, et al. 2014. Front Oncol. 4:132.
4. Hart GW, et al. 2014. Cell Metab. 20:208. - Gene ID
- 8473 View all products for this Gene ID
- UniProt
- View information about O-GlcNAc on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All O-GlcNAc Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-O-GlcNAc | RL2 | WB,ICC,IHC,IP |
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Purified anti-O-GlcNAc
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