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Purified anti-P2RX7 Antibody

Pricing & Availability
Clone
W15180A (See other available formats)
Regulatory Status
RUO
Other Names
Purinergic receptor P2X 7, P2X7, P2Z receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
W15180A_PURE_P2RX7_Antibody_WB_09152016
Total cell lysate from human HeLa cells (lane 1), U87-MG cells (lane 2), and RPMI8226 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-P2RX7 (clone W15180A) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
  • W15180A_PURE_P2RX7_Antibody_WB_09152016
    Total cell lysate from human HeLa cells (lane 1), U87-MG cells (lane 2), and RPMI8226 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-P2RX7 (clone W15180A) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection. Purified anti-GAPDH (clone Poly6314) antibody was used as a loading control.
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691002 100 µg 203€
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Description

P2X purinoceptor 7 (P2RX7) is a member of the purinergic receptor P2X family. P2 purinergic receptors are cell surface receptors that are involved in proliferation of neural stem cells and have been implicated in learning, memory, and sleep. P2X receptors are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP binding. Functional P2X receptors are homo- or heterotrimeric complex composed of the P2X family members. P2RX7 functions as a ligand-gated cation channel and is responsible for synaptic transmission and ATP-dependent lysis of macrophages through the formation of membrane pores. P2RX7 is highly expressed in hematopoietic cells and is involved in cell death, host defense against pathogens, and the regulation of inflammatory responses. P2RX7 is also found in the nervous system, microglia, uterine endometrium, and the retina. Mutations in the P2RX7 gene have been associated to major depressive disorder (MDD) and low bone mineral density.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human P2RX7 recombinant protein (43-334 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2629827 (BioLegend Cat. No. 691002)

Antigen Details

Structure
595 amino acids with a predicted molecular weight of 68.3 kD. Multi-pass transmembrane protein.
Distribution
Cell membrane and the cytoplasm.
Function
P2RX7 is a ligand-gated cation channel that participates in synaptic transmission and the regulation of immune responses.
Interaction
Forms a homotrimer. Interacts with LAMA3, ITGB2, ACTB, ACTN4, SVIL, MPP3, HSPA1, HSPCB, HSPA8, PIK230, EMP2, and PTPRB.
Ligand/Receptor
ATP.
Biology Area
Cell Biology, Neuroscience Cell Markers, Signal Transduction
Antigen References

1. Di Virgilio F. 2015. Mol. Cell Oncol. 3:e1010937.
2. Sinadinos A, et al. 2015. PLoS Med. 12:e1001888.
3. Young CN, et al. 2015. Autophagy 11:113.
4. Gavala ML, et al. 2013. J. Leuko. Biol. 94:759.
5. Notomi S, et al. 2011. Am. J. Pathol. 179:2798.
6. Lucae S, et al. 2006. Hum. Mol. Genet. 15:2438.

Gene ID
5027 View all products for this Gene ID
UniProt
View information about P2RX7 on UniProt.org
Go To Top Version: 1    Revision Date: 09/16/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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