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Purified anti-SARS-CoV-2 S Protein S1 Recombinant Antibody

Pricing & Availability
Clone
AM004414 (See other available formats)
Regulatory Status
RUO
Other Names
Spike glycoprotein, S glycoprotein (E2)
Isotype
Human IgG1, κ
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Product Citations
publications
AM004414_PURE_SARS-CoV2_Antibody_RECOM_100920
A. Whole cell extracts (15 µg total protein) from HeLa cells mixed with the indicated amount of His-tagged recombinant SARS-CoV-2 S Protein S1 (Cat. No. 792906) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-SARS-CoV-2 S Protein S1 recombinant antibody (clone AM004414) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-human IgG antibody at a 1:3000 dilution. Purified anti-His Tag antibody (clone J095G46) was used as a control antibody. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • AM004414_PURE_SARS-CoV2_Antibody_RECOM_100920
    A. Whole cell extracts (15 µg total protein) from HeLa cells mixed with the indicated amount of His-tagged recombinant SARS-CoV-2 S Protein S1 (Cat. No. 792906) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) purified anti-SARS-CoV-2 S Protein S1 recombinant antibody (clone AM004414) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-human IgG antibody at a 1:3000 dilution. Purified anti-His Tag antibody (clone J095G46) was used as a control antibody. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • AM004414_PURE_SARS-CoV2_Antibody_RECOM_2_100920
    Recombinant SARS-CoV-2 S Protein S1 (Cat. No. 792904) coated onto Nunc™ Maxisorp™ ELISA Plates (Cat. No. 423501) at 2 µg/mL and then incubated with a dilution series of purified anti-SARS-CoV-2 S Protein S1 recombinant antibody (clone AM004414). Bound antibodies were detected with biotinylated anti-human IgG secondary antibody followed by avidin-horseradish peroxidase and TMB substrate solution. Absorbance was measured at 450 nm.
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940401 25 µg 137€
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940402 100 µg 465€
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Description

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a single- stranded RNA virus that belongs in a family of viruses known as coronaviruses.   SARS-CoV-2 infection, known as COVID-19, was declared a pandemic by WHO on March 11, 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins. The spike glycoprotein (S) is a transmembrane molecule that forms homotrimers on the surface of the virus and facilitates virulence through binding to angiotensin-converting enzyme 2 (ACE2) expressed on host cells. This protein is composed of two subunits S1 and S2. S1 subunit is responsible for binding to the host cell via ACE-2 receptor and S2 for fusion with the host cell.

This antibody is specific for the S1 subunit of SARS-CoV-2 and was derived, sequenced and expressed from patients that had recovered from COVID-19 infection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
SARS-CoV-2
Antibody Type
Recombinant
Host Species
Human
Immunogen
SARS-CoV-2
Formulation
140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Prior to use, quick spin the antibody vial to ensure recovery of maximum volume. Antibody can be stored in single use aliquots in -20°C for 2 years. Avoid repeated freeze and thaws.
Application

WB - Quality tested
Direct ELISA - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. Recommended usage concentration for direct ELISA application is 93.6 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application. 

Application Notes

Clone name AM004414 is also referred to as clone 414-4.

Application References

1. Wan J. et al. 2020. Cell Rep. 32(3):107918

RRID
AB_2888747 (BioLegend Cat. No. 940401)
AB_2888747 (BioLegend Cat. No. 940402)

Antigen Details

Structure
Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 a.a with a molecular weight of approximately 141 kD and consists of S1 and S2 subunits. S1 subunit is 673 a.a. long.
Distribution

Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane

Function
Host cell surface receptor binding
Interaction
ACE2
Ligand/Receptor
ACE2
Biology Area
COVID-19
Antigen References

1. Walls A.C. et al. 2020. Cell 181(2):281-292
2. Yan R. et al. 2020 Science 367 (6485):1444-1448
3. Wrapp D. et al. 2020 Science 367 (6483):1260-126
4. Shang J.et al. 2020 PNAS 117(21):11727-11734

Gene ID
43740568 View all products for this Gene ID
UniProt
View information about Viral Protein on UniProt.org
Go To Top Version: 1    Revision Date: 09/21/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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