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Purified anti-SMARCA2 (BRM) Antibody

Pricing & Availability
Clone
W19134A (See other available formats)
Regulatory Status
RUO
Other Names
HBRM, BRM, Probable Global Transcription Activator SNF2L2, ATP-Dependent Helicase SMARCA2, BRG1-Associated Factor 190B, Protein Brahma Homolog, Brahma Homolog, SNF2-Alpha, BAF190B, BAF190, SNF2L2, SNF2LA, HSNF2a, Sth1p, SNF2, SW12
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W19134A_PURE_SMARCA2_Antibody_020821
Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL purified anti-SMARCA2 (BRM) antibody (clone W19134A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10,000 dilution (lower). Lane M: Molecular weight marker.
  • W19134A_PURE_SMARCA2_Antibody_020821
    Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 μg/mL purified anti-SMARCA2 (BRM) antibody (clone W19134A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10,000 dilution (lower). Lane M: Molecular weight marker.
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941501 25 µg 95€
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941502 100 µg 235€
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Description

SMARCA2 (also known as BRM) is a subunit of the SWI/SNF complex that facilitates chromatin remodeling. As a catalytic subunit, SMARCA2 contains an ATPase domain which uses ATP to alter DNA-histone interactions within a nucleosome. SMARCA2 as part of the chromatin remodeling complex regulates the expression of genes involved in cell cycle, metabolism, development and cancer. In most cases of cancer there is either downregulation or deficiency of the BRM gene. SMARCA2 serves as a target for anticancer therapy. Mutations in SMARCA2 can result in Nicolaides-Baraitser syndrome.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human SMARCA2 peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100. Neither method was compatible with SMARCA2 staining.

This clone failed to enrich SMARCA2 from HeLa extracts when tested for IP.

RRID
AB_2890859 (BioLegend Cat. No. 941501)
AB_2890859 (BioLegend Cat. No. 941502)

Antigen Details

Structure
SMARCA2 is a 1,590 amino acid protein with a predicted molecular weight of 181 kD
Distribution

Ubiquitously expressed/Nucleus

Function
Chromatin Remodeling
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics
Antigen References

1. Boris G. et al., 2014. Molecular and Cellular Biology. 34(6) 1136-1144
2. Euskirchen G. et al., 2012. J Biol Chem. 287(27) 30897-905
3. Van Houdt, J. et al., 2012. Nat Genet. 44 445-449
4. Vangamudi B. et al., 2015. Cancer Res. 75(18) 3865-3878

Gene ID
6595 View all products for this Gene ID
UniProt
View information about SMARCA2 on UniProt.org
Go To Top Version: 1    Revision Date: 02/08/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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