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- Clone
- BL26882 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Recombinant Strep tag fused human Her2 (23-652 aa) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti- Strep-Tag (clone BL26882) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 688202 | 25 µg | 72€ | ||||
The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
Product DetailsProduct Details
- Verified Reactivity
- Epitope tag
- Reported Reactivity
- Species independent
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic Peptide
- Formulation
- PBS pH: 7.4, containing 0.5 % BSA, 0.02% sodium azide, and 50% glycerol.
- Preparation
- The antibody was affinity purified from mouse ascites by affinity chromatography using specific immunogen.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2629594 (BioLegend Cat. No. 688202)
Antigen Details
- Structure
- The amino acid sequence of the Strep-tag is: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys.
- Function
- Strep-tag can be attached to the N- or C-terminus of protein to become Strep-tag fused protein.
- Biology Area
- Cell Biology
- Antigen References
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1. Skerra A, et al. 2000. Methods Enzymol. 326:271.
2. Schmidt TG, et al. 2007. Nat. Protoc. 2:1528. - Gene ID
- NA
- UniProt
- View information about Strep-Tag on UniProt.org
Other Formats
View All Strep-Tag Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Strep-Tag | BL26882 | WB |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Strep-Tag
Recombinant Strep tag fused human Her2 (23-652 aa) was reso...
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