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Purified anti-Strep-Tag Antibody

Pricing & Availability
Clone
BL26882 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
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Product Citations
publications
BL26882_PURE_Strep-tag_Antibody_1_WB_092216
Recombinant Strep tag fused human Her2 (23-652 aa) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti- Strep-Tag (clone BL26882) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • BL26882_PURE_Strep-tag_Antibody_1_WB_092216
    Recombinant Strep tag fused human Her2 (23-652 aa) was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti- Strep-Tag (clone BL26882) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
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688202 25 µg 72€
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Description

The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic Peptide
Formulation
PBS pH: 7.4, containing 0.5 % BSA, 0.02% sodium azide, and 50% glycerol.
Preparation
The antibody was affinity purified from mouse ascites by affinity chromatography using specific immunogen.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Wang WA, et al. 2023. J Cell Sci. 136:. PubMed
  2. Liu Y, et al. 2021. MBio. 12:e0233521. PubMed
  3. Dominguez Andres A, et al. 2020. bioRxiv. . PubMed
  4. Xu Z, et al. 2022. Front Immunol. 13:1047922. PubMed
  5. Xu Z, et al. 2021. Cell Death Dis. 12:57. PubMed
RRID
AB_2629594 (BioLegend Cat. No. 688202)

Antigen Details

Structure
The amino acid sequence of the Strep-tag is: Trp-Ser-His-Pro-Gln-Phe-Glu-Lys.
Function
Strep-tag can be attached to the N- or C-terminus of protein to become Strep-tag fused protein.
Biology Area
Cell Biology
Antigen References

1. Skerra A, et al. 2000. Methods Enzymol. 326:271.
2. Schmidt TG, et al. 2007. Nat. Protoc. 2:1528.

Gene ID
NA
UniProt
View information about Strep-Tag on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 09/20/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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