- Clone
- W16168A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transcription factor 12, Class B basic helix-loop-helix protein 20 (bHLHb20), HEB, HTF4
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
698701 | 25 µg | 81€ | ||||
698702 | 100 µg | 203€ |
TCF12, also known as HTF4 or HEB, is a member of class I basic helix-loop-helix (bHLH) protein family that recognizes the consensus E-box (5'-CANNTG-3') DNA binding site. TCF12 is a transcription factor that participates in regulating gene expression through formation of homodimer or heterodimers with other bHLH E-proteins. TCF12 has been shown to be involved in neuron differentiation and is upregulated in certain cancer cells. TCF12 functions as a transcriptional repressor responsible for down-regulating expression of E-cadherin in colorectal cancer cells. TCF12-deficient mice show abnormality in brain development and a high percentage of postnatal death. Mutations in TCF12 gene have been linked to coronal craniosynostosis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human TCF12 recombinant protein (108-521 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 2.0 µg per ml (1:250-1:2500 diluted). It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2687319 (BioLegend Cat. No. 698701)
AB_2687319 (BioLegend Cat. No. 698702)
Antigen Details
- Structure
- 706 amino acids with a predicted molecular weight of 76 kD. Contains a C-terminal bHLH domain responsible for DNA binding.
- Distribution
-
Nucleus
- Function
- TCF12 is a transcriptional regulator involved in neuronal development and is up-regulated in some type of tumors.
- Interaction
- Forms a homodimer or a heterodimer with myogenin, E12 and ITF2 proteins. Interacts with PTF1A, NEUROD2, RUNX1T1, AML1-MTG8/ETO, and BHLHA9.
- Biology Area
- Cell Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Yi S, et al. 2017. Stem Cells. 35:386.
2. Chen YF, et al. 2016. Cancer Res. 76:4872.
3. Goos JA, et al. 2016. Hum. Mutat. 37:732.
4. Labreche K, et al. 2015. Nat. Commun. 6:7207.
5. Sharma VP, et al. 2013. Nat. Genet. 45:304.
6. Lee CC, et al. 2012. J. Biol. Chem. 287:2798. - Gene ID
- 6938 View all products for this Gene ID
- UniProt
- View information about TCF12 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All TCF12 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-TCF12 | W16168A | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-TCF12
Total cell lysate (15µg protein) from MCF-7 (negative contro...
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