- Clone
- W15147A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 7B (ZBTB7B), Krueppel-related zinc finger protein (cKrox), Zinc finger protein 67 homolog (ZFP-67), Thpok
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
688302 | 100 µg | 212€ |
Th-POK is a member of the BTB-POZ domain-containing zinc finger transcription factor family. It regulates gene expression during intrathymic T cell differentiation. A single autosomal recessive mutation of the Th-POK gene results in the absence of mature CD4+ helper T cells, called helper-deficient (HD) phenotype. In Th-POK deficient mice, MHC class II restricted thymocytes are redirected to the CD8+ T cell lineage. On the contrary, forced expression of Th-POK redirects MHC class I restricted thymocytes to the CD4+ T cell lineage. Inactivation of Th-POK in mature CD4+ T cells results in induction of the genes preferentially expressed in CD8+ T cells, such as CD8, granzyme B, and IFN-γ. These findings suggest that Th-POK is essential in promoting commitment of immature T cells to the CD4 lineage and maintaining the characteristics of mature CD4+ T helper cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial mouse Th-POK recombinant protein (197-350 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg/ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone recognizes mouse TH-POK.
This clone displayed a similar affinity to BioLegend clone 11H11A14 by western blot. However, clone 11H11A14 does not recognize mouse TH-POK. - RRID
-
AB_2617025 (BioLegend Cat. No. 688302)
Antigen Details
- Structure
- 539 amino acids with a predicted molecular weight of 58 kD and observed of approximately 70 kD; contains four C2H2-type zinc finger regions that are responsible for DNA binding; contains a BTB-POZ domain which is involved in homodimerization.
- Distribution
-
Nucleus.
- Function
- Transcription factor that regulates CD4 lineage commitment of immature T-cell precursors; acts as a transcriptional repressor of collagen and fibronectin genes.
- Interaction
- Interacts with the acetyltransferase p300.
- Biology Area
- Cell Biology, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Kappes DJ. 2010. Immunol. Rev. 238:182.
2. Mariani F, et al. 2013. PLoS One 8:e54488.
3. Zhang M, et al. 2010. J. Immunol. 185:3960.
4. Egawa T, et al. 2008. Nat. Immunol. 9:1131.
5. Wildt KF, et al. 2007. J. Immunol. 179:4405. - Gene ID
- 51043 View all products for this Gene ID
- UniProt
- View information about Th-POK on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Th-POK (ZFP-67) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Th-POK (ZFP-67) | W15147A | WB,ICC |
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