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Purified Mouse IgG2b, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
27-35 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
27-35_GoChIP_IgG2b-k_ctrl_1_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone 27-35, Cat. No. 402211) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MDM2 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 27-35_GoChIP_IgG2b-k_ctrl_1_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone 27-35, Cat. No. 402211) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MDM2 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 27-35_GoChIP_IgG2b-k_ctrl_2_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 HaCaT cells with either A) 1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 (clone W15093A, Cat. No. 687203) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2b, κ isotype control antibody (clone 27-35, Cat. No. 402211) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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Cat # Size Price Quantity Check Availability Save
402201 50 µg 38€
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402202 500 µg 104€
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Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Dansyl-Keyhole Limpet Hemocyanin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Product Citations
  1. Yang A, et al. 2023. J Immunother Cancer. 11:. PubMed
  2. El-Husseiny HM, et al. 2023. Int J Mol Sci. 24:. PubMed
  3. Poggi A, et al. 2022. Curr Protoc. 2:e366. PubMed
  4. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
RRID
AB_2936439 (BioLegend Cat. No. 402201)
AB_2936439 (BioLegend Cat. No. 402202)

Antigen Details

Gene ID
NA
Go To Top Version: 2    Revision Date: 08/24/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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