Recombinant Human Frizzled-5-Fc Chimera (carrier-free)

Product Details

Source

Recombinant human Frizzled-5, amino acid Ala27-Trp238 (Accession No. Q13467) with a C-terminal human IgG1 (Pro100-Lys330) Fc tag was expressed in CHO cells.

Molecular Mass

The 451 amino acid recombinant protein has a predicted molecular mass of approximately 50.5 kD. The DTT-reduced and non-reduced proteins migrate at approximately 62 - 80 kD and 100 - 140 kD by SDS-PAGE respectively. The N-terminal amino acid is Ala.

Purity

> 95%, as determined by Coomassie stained SDS-PAGE.

Formulation

0.22 µm filtered protein solution is in PBS, pH 6.8

Endotoxin Level

Less than 0.1 EU per µg protein as determined by the LAL method.

Concentration

50 µg at 200 µg/mL

Storage & Handling

Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Activity

ED₅₀ = 0.08 – 0.64 µg/mL as measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells induced by 50 ng/mL of recombinant mouse Wnt3a.

Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure

Homodimer

Distribution

Expressed in adult retina, colon, and pancreatic islets, human embryonic stem cells and embryonic tissues (telencephalon, pituitary, thalamus, hypothalamus, eye, liver, spleen, lung, and kidney)

Function

Receptor for Wnt proteins that functions in the canonical Wnt/beta-catenin signaling pathway

Ligand/Receptor

Wnt3a, Wnt3, Wnt5a, Wnt7a, Wnt9b, Wnt11, and Wnt1

Bioactivity

Measured by its ability to inhibit Wnt3a activity in Topflash assay

Cell Type

Embryonic Stem Cells, Lymphocytes, Monocytes

Biology Area

Cell Biology, Immuno-Oncology, Immunology, Neuroscience, Signal Transduction, Stem Cells

Molecular Family

Fc Receptors, Soluble Receptors

Antigen References

1. Teo S, et al. 2023. Dev Cell. 58:2063-2079.
2. Calandria JM, et al. 2023. Cell Mol Neurobiol. 43:1077-1096.
3. Range RC. 2018. Dev Biol. 444:83-92.
4. Van Raay TJ, et al. 2005. Neuron. 46:23-36.
5. Liu C and Nathans J. 2008. Development. 135:3567-76.
6. Sahores M, et al. 2010. Development. 137:2215-25.

Gene ID

7855 View all products for this Gene ID

UniProt

View information about Frizzled-5 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Certificate of Analysis