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- Regulatory Status
- RUO
- Other Names
- CD200 receptor 1, CD200R, CRTR2, MOX2 receptor, MOX2R, OX2 receptor, OX-2 receptor, OX2R
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- Product Citations
- publications
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When mouse CD200R1-Fc Chimera is immobilized at 0.5 µg/mL, biotinylated mouse CD200 binds with EC50 of 6 – 24 ng/mL. -
Stability testing for Mouse CD200R1-Fc Chimera. Mouse CD200R1-Fc Chimera was aliquoted in PBS, pH 7.2 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw), and compared to a control kept at 4⁰C (Control). The samples were tested in a binding assay with biotinylated mouse CD200.
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CD200R1, also known as OX-2 receptor, is an Ig superfamily transmembrane glycoprotein found in myeloid cells and also can be induced in certain T-cell subsets. CD200R1 is composed of a 215 amino acid extracellular domain, a 21 amino acid transmembrane segment, and a 61 amino acid cytoplasmic domain. CD200R1 interacts with CD200 which is also an Ig superfamily transmembrane glycoprotein to downregulate myeloid cell functions. The CD200R1 extracellular domain contains two IgSF domains and the cytoplasmic domain contains three tyrosine residues, one of which forms part of an NPXY motif. CD200R1 expression is restricted primarily to mast cells, basophils, macrophages, and dendritic cells, which suggests myeloid cell regulation as the major function of the CD200:CD200R1 axis. CD200 and CD200R interact with each other through N-terminal IgSF domains. Their interaction plays important roles in negatively regulating immune responses, and attenuating autoimmune diseases and excessive inflammatory responses against pathogens. High expression of CD200 in the CNS is thought to be a mechanism of constitutive immune suppression, and is developmentally regulated in the mouse brain. Deficiency in neuronal CD200 may explain the chronic inflammation in human neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and multiple sclerosis. The CD200 and CD200R interaction has also been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in a number of malignancies, including CLL, as well as on cancer stem cells.
Product DetailsProduct Details
- Source
- Mouse CD200R1, amino acid Thr26-Pro238 (Accession # Q9ES57) with a C-terminal human IgG Fc tag, was expressed in CHO cells.
- Molecular Mass
- The 452 amino acid recombinant protein has a predicted molecular mass of approximately 50 kD. The DTT-reduced proteins migrate at approximately 70 kD, and non-reduced proteins migrate at approximately 140 kD by SDS-PAGE. The predicted N-terminal amino acid is Thr.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 μm filtered protein solution is in PBS, pH7.2
- Endotoxin Level
- Less than 0.1 EU per µg protein as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- When mouse CD200R1-Fc Chimera is immobilized at 0.5 µg/mL, biotinylated mouse CD200 binds with EC50 of 6 – 24 ng/mL
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Disulfide-linked homodimer
- Distribution
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Mast cells, basophils, macrophages, and dendritic cells
- Function
- Inhibitory receptor for the CD200. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli.
- Interaction
- Neurons and vascular endothelium in CNS, and thymocytes, T and B cells, and dendritic cells.
- Ligand/Receptor
- CD200
- Cell Type
- Macrophages, Mast cells
- Biology Area
- Immunology, Costimulatory Molecules, Inhibitory Molecules
- Molecular Family
- Soluble Receptors
- Antigen References
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- Hernangómez M, et al. 2014. Curr Pharm Des. 20:4707-22.
- Walker DG and Lue LF. 2013. Future Neurol. 10.2217/fnl.13.14.
- Wright GJ, et al. 2003. J Immunol. 171: 3034-46.
- Vaine CA and Soberman RJ. 2014. Adv Immunol. 121:191-211.
- Minas K and Liversidge J. 2006. Crit Rev Immunol. 26(3): 213-30.
- Wang XJ, et al. 2007. J Neuroimmune Pharmacol. 2(3): 259-64.
- Wong KK, et al. 2010. J Leukoc Biol. 88(2): 361-72.
- Gene ID
- 57781 View all products for this Gene ID
- UniProt
- View information about CD200R1 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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