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Recombinant Mouse IL-10 (ELISA Std.)

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin-10, Cytokine synthesis inhibitory factor (CSIF), B cell derived T cell growth factor (B-TCGF), T cell growth inhibitory factor (TGIF)
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Product Citations
publications
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575809 4 pack 76€
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Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. Recombinant mouse IL-10 is a 18.5 kD protein containing 160 amino acids.

Product Details
Technical Data Sheet (pdf)

Product Details

Formulation
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1% BSA, 0.09% sodium azide, and protease inhibitors.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
Unopened vials can be stored between 2°C and 8°C until the expiration date. Prior to use, reconstitute the lyophilized powder with 0.2 mL of PBS containing a carrier protein (e.g., 1% BSA, protease free), pH7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70°C for up to one month. Do not re-use diluted standards. Avoid repeated freeze/thaw cycles.
Application

ELISA
Recommended Usage

ELISA with capture antibody clone JES5-2A5 (Cat. No. 504901) or clone MK10A6 (Cat. No. 521801) and detection antibody clone JES5-16E3 (Cat. No. 505003), with an approximate standard range from 30 pg/mL to 4 ng/mL.

Product Citations
  1. Vázquez-Zapién GJ, et al. 2022. Cells. 11: . PubMed

Antigen Details

Structure
Acid-labile cytokine; dimer
Bioactivity
Inhibits IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppresses delayed type hypersensitivity response; stimulates TH2 cell response; mast cell proliferation i
Cell Sources
Activated CD8+ T cells, TH0, TH2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
Cell Targets
T cells, B cells, mast cells, macrophages
Receptors
IL-10R (CDw210)
Biology Area
Immunology, Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385.

Regulation
Downregulated by IL-4, IL-10
Gene ID
16153 View all products for this Gene ID
UniProt
View information about IL-10 on UniProt.org
Go To Top Version: 2    Revision Date: 02/29/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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