Spark Red™ 718 anti-human CD138 (Syndecan-1) (Flexi-Fluor™) Antibody, CD138, MI15

Pricing & Availability

Clone: MI15 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B-B4
Isotype: Mouse IgG1, κ
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Product Citations: publications

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285093

50 µg

300€

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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells, but not on mature circulating B-lymphocytes. It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial cells, and neural cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: A mixture of U266 and XG-1 human myeloma cell lines.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
Concentration: 0.2 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC
Recommended Usage: Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
Excitation Laser: Red Laser (633 nm)
Application Notes: The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138⁺ cell lines.⁴

Additional reported applications for the relevant formats include: immunofluorescent staining¹, Western blotting², immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections³, and spatial biology (IBEX)^5,6.
Additional Product Notes: For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
RRID: AB_3106348 (BioLegend Cat. No. 285093)

Antigen Details

Structure: 100-200 kD type I integral transmembrane glycoprotein
Distribution: Plasma cells, pre-B cells, epithelial cells, endothelial cells
Function: Adhesion, controls cell morphology, regulates cell growth
Ligand/Receptor: FGFb, collagen, fibronectin
Cell Type: B cells, Endothelial cells, Epithelial cells, Plasma cells
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sanderson RD, et al. 1992. Cell. Regul. 1:27.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers. Wiley-Liss A John Wiley & Sons Inc, Publication.
Gene ID: 6382 View all products for this Gene ID
UniProt: View information about CD138 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

B cell Development and Activation

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 10⁷ cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance.

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool.

Other Formats

View All CD138 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD138 (Syndecan-1)	MI15	FC,SB
Purified anti-human CD138 (Syndecan-1)	MI15	FC,ICC,WB,IHC-P,SB
APC anti-human CD138 (Syndecan-1)	MI15	FC
FITC anti-human CD138 (Syndecan-1)	MI15	FC,SB
PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1)	MI15	FC
Alexa Fluor® 700 anti-human CD138 (Syndecan-1)	MI15	FC
PE/Cyanine7 anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 421™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 510™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 605™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 711™ anti-human CD138 (Syndecan-1)	MI15	FC
Alexa Fluor® 647 anti-human CD138 (Syndecan-1)	MI15	FC,SB
Alexa Fluor® 594 anti-human CD138 (Syndecan-1)	MI15	ICC
PE/Dazzle™ 594 anti-human CD138 (Syndecan-1)	MI15	FC
APC/Cyanine7 anti-human CD138 (Syndecan-1)	MI15	FC
Pacific Blue™ anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-A0055 anti-human CD138 (Syndecan-1)	MI15	PG
Brilliant Violet 785™ anti-human CD138 (Syndecan-1)	MI15	FC
Biotin anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-C0055 anti-human CD138 (Syndecan-1)	MI15	PG
APC/Fire™ 750 anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-B0055 anti-human CD138 (Syndecan-1)	MI15	PG
PE/Cyanine5 anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-D0055 anti-human CD138 (Syndecan-1)	MI15	PG
PE/Fire™ 640 anti-human CD138 (Syndecan-1)	MI15	FC
Spark Violet™ 500 anti-human CD138 (Syndecan-1)	MI15	FC
FITC anti-human CD138	MI15	FC
PE/Fire™ 700 anti-human CD138 (Syndecan-1)	MI15	FC
Spark Violet™ 423 anti-human CD138 (Syndecan-1)	MI15	FC
Spark Red™ 718 anti-human CD138 (Syndecan-1) (Flexi-Fluor™)	MI15	FC
GMP FITC anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 650™ anti-human CD138 (Syndecan-1)	MI15	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 02/07/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.