- Clone
- ML5 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V CD24.5
- Other Names
- Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- Application References
-
- Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
- Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
Antigen Details
- Structure
- GPI-linked glycoprotein, 35-45 kD
- Distribution
-
B cells, granulocytes, epithelial cells
- Function
- B cell proliferation and differentiation
- Ligand/Receptor
- CD62P (P-Selectin)
- Cell Type
- B cells, Epithelial cells, Granulocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
- Gene ID
- 100133941 View all products for this Gene ID
- UniProt
- View information about CD24 on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD24 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-human CD24
Human peripheral blood lymphocytes stained ML5 FITC -
PE anti-human CD24
Human peripheral blood lymphocytes stained ML5 PE -
Purified anti-human CD24
Human whole blood lymphocytes stained with purified ML5 and ... MCF-7 breast cancer cells were stained with anti-CD24 (clone... -
Alexa Fluor® 488 anti-human CD24
Human peripheral blood lymphocytes stained ML5 Alexa Fluor&r... -
Alexa Fluor® 647 anti-human CD24
Human peripheral blood lymphocytes stained ML5 Alexa Fluor® ... MDA-MB231 breast cancer cell line was stained with 10 µg/mL ... -
PerCP/Cyanine5.5 anti-human CD24
Human peripheral lymphocytes were stained with CD24 (clone M... -
APC anti-human CD24
Human peripheral blood lymphocytes stained with ML-5 APC -
PE/Cyanine7 anti-human CD24
Human peripheral blood lymphocytes stained with ML-5 PE/Cyan... -
Brilliant Violet 421™ anti-human CD24
Human peripheral blood lymphocytes stained with CD19 APC and... -
Brilliant Violet 605™ anti-human CD24
Human peripheral blood lymphocytes were stained with CD19 AP... -
PerCP anti-human CD24
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 510™ anti-human CD24
Human peripheral blood lymphocytes stained with CD19 APC and... -
Purified anti-human CD24 (Maxpar® Ready)
Human NALM-6 pre-B cells (top) and human Jurkat T cells (bot... -
Biotin anti-human CD24
Human peripheral blood lymphocytes were stained with CD19 AP... -
APC/Cyanine7 anti-human CD24
Human peripheral blood lymphocytes were stained with CD19 A... -
PE/Dazzle™ 594 anti-human CD24
Human peripheral blood lymphocytes were stained with CD19 AP... -
Brilliant Violet 711™ anti-human CD24
Human peripheral blood lymphocytes were stained with CD19 PE... -
PE anti-human CD24
Typical results from human peripheral blood lymphocytes stai... -
TotalSeq™-A0180 anti-human CD24
-
APC/Fire™ 750 anti-human CD24
Human peripheral blood lymphocytes were stained with CD19 Br... -
Brilliant Violet 785™ anti-human CD24
Human peripheral blood lymphocytes stained with CD19 FITC an... -
TotalSeq™-C0180 anti-human CD24
-
TotalSeq™-B0180 anti-human CD24
-
PE/Cyanine5 anti-human CD24
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0180 anti-human CD24
-
GMP PE anti-human CD24
Typical results from human peripheral blood lymphocytes stai... -
APC/Fire™ 750 anti-human CD24
Typical results from human peripheral blood lymphocytes stai... -
Brilliant Violet 650™ anti-human CD24
Human peripheral blood lymphocytes were stained with anti-hu... -
PerCP/Cyanine5.5 anti-human CD24
Typical results from human peripheral blood lymphocytes stai... -
Spark Blue™ 550 anti-human CD24 (Flexi-Fluor™)
-
Spark Red™ 718 anti-human CD24 (Flexi-Fluor™)
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