- Clone
- ME20.4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, NGFR, Nerve Growth Factor Receptor
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
CD271, also known as p75NTR, TNFRSF16, p75(NTR), Gp80-LNGFR, and NGFR, is a type I transmembrane protein with a MW of 75 kD. It is expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells and melanocytes. The extracellular portion contains four TNFR-Cys repeats that form the binding domain for its ligands (NGF, BDNF, NTF3, and NTF4). The intracellular portion of CD271 contains a death domain, which interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9, to promote cell apoptosis, and to regulate cell differentiation and neurogenesis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- WM245 melanoma cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications for the relevant formats include: immunoprecipitation1, Western blotting4, immunohistochemical staining of frozen or paraffin embedded tissue sections1,2, and blocking of NGF binding1.
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- Application References
-
- Ross A, et al. 1984. P. Natl. Acad. Sci. USA 81:6681. (FC, IP, Block, IHC)
- Cattoretti G, et al. 1993. Blood 81:1726. (IHC)
- Kamke MR, et al. 2005. Hear Res. 206:89.
- Baker D, et al. 1989. Cancer Res. 49:4142-4146. (FC, WB)
- RRID
-
AB_3662437 (BioLegend Cat. No. 285201)
Antigen Details
- Structure
- Type I transmembranal protein with a MW of 75 kD. The extracellular portion contains four TNFR-Cys repeats that form the NGF binding domain; the intracellular portion contains a death domain.
- Distribution
-
Expressed by many cell types including neurons, Schwann cells, mesenchymal stem/stromal cells, follicular dendritic cells, melanocytes.
- Function
- Apoptosis, differentiation, neurogenesis.
- Interaction
- TRAF2, TRAF4, TRAF6, PTPN13, RANBP9.
- Ligand/Receptor
- Nerve growth factor (NGF), Brain-derived neurotrophic factor (BDNF), Neurotrophin 3. (NTF3) , Neurotrophin 4 (NTF4)
- Bioactivity
- Apoptosis, differentiation, neurogenesis.
- Cell Type
- Dendritic cells, Mesenchymal cells, Mesenchymal Stem Cells, Neurons
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, Postsynaptic proteins
- Antigen References
-
1. Friedman MJ, et al. 2009. J. Biol. Chem. 284:27944.
2. Rock JR, et al. 2009. Proc Natl Acad Sci USA. 106:12771.
3. Kidd SK, et al. 2008. Brain Res.1195:113.
4. Peters EM, et al. 2007. Am J Pathol. 171:1872.
5. Jansen P, et al. 2007. Nat Neurosci. 10:1449. - Gene ID
- NA
- UniProt
- View information about CD271 on UniProt.org
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD271 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human CD271 (NGFR) | ME20.4 | FC,IP,WB,IHC-P,IHC-F |
FITC anti-human CD271 (NGFR) | ME20.4 | FC |
PE anti-human CD271 (NGFR) | ME20.4 | FC |
APC anti-human CD271 (NGFR) | ME20.4 | FC |
PE/Cyanine7 anti-human CD271 (NGFR) | ME20.4 | FC |
PerCP/Cyanine5.5 anti-human CD271 (NGFR) | ME20.4 | FC |
Alexa Fluor® 647 anti-human CD271 (NGFR) | ME20.4 | FC |
Alexa Fluor® 700 anti-human CD271 (NGFR) | ME20.4 | FC |
PE/Dazzle™ 594 anti-human CD271 (NGFR) | ME20.4 | FC |
APC/Fire™ 750 anti-human CD271 (NGFR) | ME20.4 | FC |
Biotin anti-human CD271 (NGFR) | ME20.4 | FC |
TotalSeq™-A0816 anti-human CD271 (NGFR) | ME20.4 | PG |
APC/Cyanine7 anti-human CD271 (NGFR) | ME20.4 | FC |
TotalSeq™-C0816 anti-human CD271 (NGFR) | ME20.4 | PG |
TotalSeq™-B0816 anti-human CD271 (NGFR) | ME20.4 | PG |
Pacific Blue™ anti-human CD271 (NGFR) Antibody | ME20.4 | FC |
TotalSeq™-D0816 anti-human CD271 (NGFR) | ME20.4 | PG |
Spark Red™ 718 anti-human CD271 (NGFR) (Flexi-Fluor™) | ME20.4 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD271 (NGFR)
-
FITC anti-human CD271 (NGFR)
-
PE anti-human CD271 (NGFR)
-
APC anti-human CD271 (NGFR)
-
PE/Cyanine7 anti-human CD271 (NGFR)
-
PerCP/Cyanine5.5 anti-human CD271 (NGFR)
-
Alexa Fluor® 647 anti-human CD271 (NGFR)
-
Alexa Fluor® 700 anti-human CD271 (NGFR)
-
PE/Dazzle™ 594 anti-human CD271 (NGFR)
-
APC/Fire™ 750 anti-human CD271 (NGFR)
-
Biotin anti-human CD271 (NGFR)
-
TotalSeq™-A0816 anti-human CD271 (NGFR)
-
APC/Cyanine7 anti-human CD271 (NGFR)
-
TotalSeq™-C0816 anti-human CD271 (NGFR)
-
TotalSeq™-B0816 anti-human CD271 (NGFR)
-
Pacific Blue™ anti-human CD271 (NGFR) Antibody
-
TotalSeq™-D0816 anti-human CD271 (NGFR)
-
Spark Red™ 718 anti-human CD271 (NGFR) (Flexi-Fluor™)
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