Ultra-LEAF™ Purified anti-human IL-10 Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Baboon, Rhesus, Cynomolgus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

COS - expressed, recombinant human IL-10

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture - Quality tested
IHC-P, Neut - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISPOT capture antibody, a concentration range of 4.0 - 8.0 µg/ml is recommended. For ELISA capture applications, a concentration range of 2.0 - 6.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 250 to 2 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture^1-5 or ELISPOT Capture⁶: The Ultra-LEAFpurified JES3-9D7 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. No. 501502) as the detecting antibody and recombinant human IL-10 (Cat. No. 571009) as the standard. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.
Neutralization^1-3,9: The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 501427 & 501428). The JES3-9D7 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Additional reported applications (for the relevant formats) include: immunohistochemical staining¹².
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. Nos. 430601 & 430606) are specially developed and recommended.

The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10).

Application References

1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Capture, Neut)
2. Gotlieb W, et al. 1992. Cytokine 4:385. (ELISA Capture, Neut)
3. Yssel H, et al. 1992. J. Immunol. 149:2378. (ELISA Capture, Neut)
4. Abrams J. 1995. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.20. (ELISA Capture)
5. Burdin N, et al. 1993. J. Exp. Med. 177:295. (ELISA Capture)
6. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Capture)
7. Schaerli P, et al. 2000. J. Exp. Med. 192:1553.
8. Jason J, et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
9. Akdis CA, et al. 1998. J. Clin. Invest. 102:98. (Neut)
10. Stary G, et al. 2011. J. Immunol. 186:103. PubMed
11. Mason GM, et al. 2012. PNAS. PubMed
12. 12. Smith DR, et al. 1994. Am. J. Pathol. 145:18. (IHC)

Product Citations

1. Gruber CN, et al. 2020. Cell. 183:982. PubMed
2. Jiang J, et al. 2021. Front Immunol. 12:785549. PubMed
3. Fu B, et al. 2021. Front Immunol. 11:591269. PubMed

RRID

AB_2814392 (BioLegend Cat. No. 501427)
AB_2814392 (BioLegend Cat. No. 501428)

Antigen Details

Structure

Acid-labile cytokine, dimer, 35-40 kD (Mammalian)

Bioactivity

Inhibit IFN-γ, TNF-β, IL-2 production by T_H1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate T_H2 cell response; mast cell proliferation in

Cell Sources

Activated CD8⁺ and CD4⁺ T cells, activated monocytes, mast cells, Ly-1 B (mouse)

Cell Targets

T cells, B cells, mast cells, macrophages

Receptors

IL-10R (CDw210)

Cell Type

Tregs

Biology Area

Cell Biology, Immunology, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385.

Regulation

Production inhibited by IL-4, IL-10

Gene ID

3586 View all products for this Gene ID

UniProt

View information about IL-10 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Material Safety Data Sheet

Reviews

Related Protocols

• Active Protocols: Sandwich ELISA - Video
• Sandwich ELISA Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis