Ultra-LEAF Purified anti-mouse CD106 Antibody, CD106, 429 (MVCAM.A)

Pricing & Availability

Clone: 429 (MVCAM.A) (See other available formats)
Regulatory Status: RUO
Other Names: VCAM-1, INCAM-110
Isotype: Rat IgG2a, κ
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Product Citations: publications

C57BL/6 mouse bone marrow myeloid cells stained with 429 purified, followed by anti-rat IgG FITC.

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105727

100 µg

141€

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105728

1 mg

542€

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Description

CD106 is a 110 kD glycosylphosphatidylinositol (GPI)-linked transmembrane protein, also known as VCAM-1 and INCAM-110. It is constitutively expressed on bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells, as well as some lymphocytes. CD106 expression can be upregulated on endothelial cells by inflammatory cytokines. CD106 is involved in adhesion and acts as a counter-receptor for VLA-4 (α₄/β₁ integrin) and LPAM-1 (α₄/β₇ integrin). The 429 antibody has been reported to partially block VCAM-1-mediated binding.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse preadipose cell line PA6
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining^2,3,5-7 of acetone-fixed frozen sections, blocking^4,5,8 of ligand binding in vitro and in vivo, immunoprecipitation¹ , and spacial biology (IBEX)^11,12. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 105727 & 105728).
RRID: AB_2819809 (BioLegend Cat. No. 105727)
AB_2819809 (BioLegend Cat. No. 105728)

Antigen Details

Structure: Ig superfamily, 47 kD
Distribution: Bone marrow stromal cells, myeloid progenitors, splenic dendritic cells, activated endothelial cells
Function: Adhesion
Ligand/Receptor: VLA-4 (α₄/β₁ integrin) and LPAM-1 (α₄/β₇ integrin)
Cell Type: Dendritic cells, Endothelial cells, Mesenchymal Stem Cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Kinashi T, et al. 1995. J. Leukoc. Biol. 57:168.
3. Bevilacquea MP. 1993. Annu. Rev. Immunol. 11:767.
4. Koni PA, et al. 2001. J. Exp. Med. 193:741.
Gene ID: 22329 View all products for this Gene ID
UniProt: View information about CD106 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD106 Reagents Request Custom Conjugation

Description	Clone	Applications
Biotin anti-mouse CD106	429 (MVCAM.A)	FC
FITC anti-mouse CD106	429 (MVCAM.A)	FC
LEAF™ Purified anti-mouse CD106	429 (MVCAM.A)	FC, IHC-F, IP
Purified anti-mouse CD106	429 (MVCAM.A)	FC,IHC-F,IP
Alexa Fluor® 488 anti-mouse CD106	429 (MVCAM.A)	FC,IHC-F,SB
Alexa Fluor® 647 anti-mouse CD106	429 (MVCAM.A)	FC,IHC-F,3D IHC
PE anti-mouse CD106	429 (MVCAM.A)	FC
PerCP/Cyanine5.5 anti-mouse CD106	429 (MVCAM.A)	FC
APC anti-mouse CD106	429 (MVCAM.A)	FC
PE/Cyanine7 anti-mouse CD106	429 (MVCAM.A)	FC
Pacific Blue™ anti-mouse CD106	429 (MVCAM.A)	FC
Alexa Fluor® 594 anti-mouse CD106	429 (MVCAM.A)	IHC-F
TotalSeq™-A0226 anti-mouse CD106	429 (MVCAM.A)	PG
Ultra-LEAF™ Purified anti-mouse CD106	429 (MVCAM.A)	FC,IHC-F,IP
TotalSeq™-C0226 anti-mouse CD106	429 (MVCAM.A)	PG
TotalSeq™-B0226 anti-mouse CD106	429 (MVCAM.A)	PG

Certificate of Analysis

Go To Top Version: 1 Revision Date: 11/15/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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