- Clone
- N2B2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TNFSF10, TNF-related apoptosis inducing ligand, Apo-2 ligand, Apo-2L, CD253
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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109315 | 100 µg | 190€ | ||||
109316 | 1 mg | 551€ |
Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD253 is a 40 kD TNF superfamily member known as TRAIL, Apo-2 ligand, and Apo-2L. TRAIL is expressed on a variety of cells, including IL-2 and IL-15 activated NK cells and activated T cells. However, it is undetectable on resting T and B cells. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. The N2B2 antibody has been reported to be useful for flow cytometric staining and blocking NK cell cytotoxicity in vitro.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse TRAIL-transfected 2PK-3 cells
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Qualit tested
Block - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: in vitro blocking of NK cell cytotoxicity1,2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109308).
- Application References
-
- Kayagaki N, et al. 1999. J. Immunol. 163:1906. (Block)
- Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
- Joshi PS, et al. 2006. J. Leukocyte Biol. 80:1345.
- Herold S, et al. 2008. J. Exp. Med. 205:3065. PubMed
- Iannello A, et al. 2009. J. Virol. 83:5999. PubMed
- Komatsu M, et al. 2003. Blood 101:3991. (Block)
- Taieb J, et al. 2006. Nature Med. 12:214. (Block)
- Gonzaga R, et al. 2011. J. Immunol. 187:6235. PubMed.
- RRID
-
AB_2813976 (BioLegend Cat. No. 109315)
AB_2813976 (BioLegend Cat. No. 109316)
Antigen Details
- Structure
- TNF ligand superfamily member, approximately 40 kD
- Distribution
-
Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells
- Function
- Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity
- Ligand/Receptor
- TRAIL receptor DR5
- Cell Type
- Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kayagaki N, et al. 1999. J. Immunol. 163:1906.
2. Wiley SR, et al. 1995. Immunity. 3:673.
3. Wu GS, et al. 1999. Cancer Res. 59:2770.
4. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973. - Gene ID
- 22035 View all products for this Gene ID
- UniProt
- View information about CD253 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD253 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse CD253 (TRAIL) | N2B2 | FC,Block |
Biotin anti-mouse CD253 (TRAIL) | N2B2 | FC |
PE anti-mouse CD253 (TRAIL) | N2B2 | FC |
APC anti-mouse CD253 (TRAIL) | N2B2 | FC |
PerCP/Cyanine5.5 anti-mouse CD253 (TRAIL) | N2B2 | FC |
PE/Cyanine7 anti-mouse CD253 (TRAIL) | N2B2 | FC |
Ultra-LEAF™ Purified anti-mouse CD253 (TRAIL) | N2B2 | FC,Block |
TotalSeq™-A0925 anti-mouse CD253 (TRAIL) | N2B2 | PG |
TotalSeq™-C0925 anti-mouse CD253 (TRAIL) | N2B2 | PG |
TotalSeq™-B0925 anti-mouse CD253 (TRAIL) | N2B2 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected cells stained with purified N2B2, fo... -
Biotin anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected cells stained with biotinylated N2B2... -
PE anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected cells stained with N2B2 PE -
APC anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected L5718Y cells were stained with CD253... -
PerCP/Cyanine5.5 anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected L5718Y cells were stained with CD253... -
PE/Cyanine7 anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected L5718Y cells were stained with CD253... -
Ultra-LEAF™ Purified anti-mouse CD253 (TRAIL)
Mouse TRAIL transfected cells stained with LEAF™ purified N2... -
TotalSeq™-A0925 anti-mouse CD253 (TRAIL)
-
TotalSeq™-C0925 anti-mouse CD253 (TRAIL)
-
TotalSeq™-B0925 anti-mouse CD253 (TRAIL)
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