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Ultra-LEAF™ Purified anti-mouse IL-13Rα2 (BA) Antibody

Pricing & Availability
Clone
W19369C (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-13 receptor subunit alpha-2, IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, IL-13R alpha-2, IL-13Rα2, IL-13Ra2, CD213a2, IL-13 binding protein, IL-13BP
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
W19369C_ULEAF_IL-13Ralpha2_Antibody_032322
Recombinant mouse IL-13Rα2-Fc chimera (Cat. No.770704) (black circles) inhibits the proliferation of human TF-1 cells induced by recombinant mouse IL-13 (Cat. No. 575902). Ultra-LEAF™ purified anti-mouse IL-13Rα2 (BA) (clone W19369C) (purple squares) neutralizes the inhibition of cell proliferation induced by recombinant mouse IL-13Rα2-Fc chimera (Cat. No.770704) at 50 ng/mL and recombinant mouse IL-13 (Cat. No. 575902) at 8 ng/mL in human TF-1 cells in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG1, κ isotype control (Cat. No. 400452) (gray triangles) does not have the effect. ND50 range: 0.01 - 0.06 μg/mL
  • W19369C_ULEAF_IL-13Ralpha2_Antibody_032322
    Recombinant mouse IL-13Rα2-Fc chimera (Cat. No.770704) (black circles) inhibits the proliferation of human TF-1 cells induced by recombinant mouse IL-13 (Cat. No. 575902). Ultra-LEAF™ purified anti-mouse IL-13Rα2 (BA) (clone W19369C) (purple squares) neutralizes the inhibition of cell proliferation induced by recombinant mouse IL-13Rα2-Fc chimera (Cat. No.770704) at 50 ng/mL and recombinant mouse IL-13 (Cat. No. 575902) at 8 ng/mL in human TF-1 cells in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG1, κ isotype control (Cat. No. 400452) (gray triangles) does not have the effect. ND50 range: 0.01 - 0.06 μg/mL
Cat # Size Price Quantity Check Availability Save
947903 100 µg 287€
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947904 1 mg 964€
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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

IL-13 is a key mediator in the pathogenesis of allergic inflammation. Its receptor is comprised of IL-4Rα and IL-13Rα1, which transduct the signaling to the downstream STAT6 pathway. IL-13Rα2, also known as IL-13Ra2 and IL-13BP, serves as a decoy receptor to attenuate IL-13-induced response. IL-13Rα2 binds IL-13 with 100-300 folds higher affinity than does IL-13Rα1, but IL-13Rα2 lacks signaling motifs on its cytoplasmic tail. Mice deficient in IL-13Rα2 showed increased IgE level and IFNγ production, consistent with a phenotype of enhanced IL-13 effects. Airway hyperresponsiveness and inflammation was enhanced in IL-13Rα2 transgene mice, indicating that IL-13Rα2 blocks IL-13 mediated response. In mice, there are two discrete transcripts generated by alternative splicing, producing membrane-bound and secreted forms, respectively. Some evidences indicated that MMP-8 contributes to soluble forms of IL-13Rα2 by cleaving membrane-bound IL-13Rα2. In human, only membrane-bound transcript was found and soluble forms were absent in human plasma.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse IL-13Rα2-Fc chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralizing the inhibition of proliferation induced by recombinant mouse IL-13Rα2-Fc chimera (Cat. No.770704) at 0.05 µg/mL on human TF-1 cells. ND50 range: 0.01-0.06 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2922648 (BioLegend Cat. No. 947903)
AB_2922648 (BioLegend Cat. No. 947904)

Antigen Details

Structure
Disulfide bond-linked homodimer
Distribution

Spleen, brain; membrane-bound and soluble form

Function
IL-13Rα2 serves as a decoy receptor to inhibit IL-13-induced responses.
Ligand/Receptor
IL-13
Cell Type
B cells, Fibroblasts
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines, Innate Immune Signaling, Soluble Receptors
Antigen References
  1. Zhang JG, et al. 1997. J Biol Chem. 272: 9474-80.
  2. Donaldson DD, et al. 1998. J Immuno. 161: 2317-24.
  3. Kawakami K, et al. 2001. Blood. 97: 2673-9.
  4. Wood N, et al. 2003. J Exp Med. 197: 703-9.
  5. Chen W, et al. 2014. J Allergy Clin Immunol. 132: 951-8.
  6. Chen W, et al. 2008. J Allergy Clin Immunol. 122: 625-32.
  7. Chen W, et al. 2009. J Immunol. 183: 7870-6.
Gene ID
16165 View all products for this Gene ID
UniProt
View information about IL-13Ralpha2 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 03/23/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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