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PE/Cyanine7 anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
EH12.2H7 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1, PDCD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_EH12dot2H7_PECy7_092809
PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 (open histogram).
  • A_EH12dot2H7_PECy7_092809
    PHA-stimulated (day-3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 (open histogram).
  • B_EH12-2H7_PECyanine7_CD279_2_Antibody_103024
    Human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) PE/Cyanine7 and CD3 (clone UCHT1) FITC.
Compare all formats See PE/Cyanine7 spectral data
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329917 25 tests 141€
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329918 100 tests 306€
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections13, and spatial biology (IBEX)15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References
  1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
  3. Velu V, et al. 2007. J. Virol. 81:5819. (FA)
  4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
  5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
  6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
  7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
  8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
  9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
  10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
  11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
  12. Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
  13. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  15. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  16. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bettini E, et al. 2022. STAR Protoc. 3:101840. PubMed
  2. Wenthe J, et al. 2021. Cancer Immunology Immunotherapy. . PubMed
  3. Zhou J, et al. 2020. Oncol Lett. 1.336111111. PubMed
  4. Mylvaganam G, et al. 2014. J Immunol. 193:4527. PubMed
  5. Amancha P, et al. 2013. J Immunol. 191:6060. PubMed
  6. Yoshitomi H, et al. 2018. Nat Commun. 2.9875. PubMed
  7. Hong J, et al. 2012. J Immunol. 188:3247. PubMed
  8. Policicchio B, et al. 2016. PLoS Pathog. 12: 1005879. PubMed
  9. Wildner NH, et al. 2022. Front Immunol. 13:886646. PubMed
  10. Olwenyi OA, et al. 2020. Cells. 9:. PubMed
  11. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  12. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  13. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  14. Tauriainen J, et al. 2017. Sci Rep. 7:40354. PubMed
  15. Au L, et al. 2021. Cancer Cell. 39:1497. PubMed
  16. Verma A, et al. 2021. Cell Rep. 37:109942. PubMed
  17. Hong J, et al. 2014. J Immunol. 193:797. PubMed
  18. Colleen S McGary et al. 2017. Immunity. 47(4):776-788 . PubMed
  19. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  20. Dart SJ, et al. 2021. Sci Rep. 11:15312. PubMed
  21. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  22. Edwards CJ, et al. 2021. Br J Cancer. . PubMed
  23. Hsu H, et al. 2016. J Immunol. 197: 1884 - 1892. PubMed
  24. Kuo HH, et al. 2018. Immunity. 48:1183. PubMed
  25. Lee J, et al. 2020. Sci Rep. 10:17753. PubMed
  26. Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed
  27. Ohue Y, et al. 2014. Clin Cancer Res. 20:5052. PubMed
  28. van Montfoort N, et al. 2018. Cell. 175:1744. PubMed
  29. Gordon-Alonso M,et al. 2017. Nat. Commun. 10.1038/s41467-017-00925-6. PubMed
  30. Parsons E, et al. 2016. PLoS One. 11:e0167841. PubMed
  31. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
  32. Den Braanker H, et al. 2021. Front Immunol. 12:768113. PubMed
  33. Beltra JC, et al. 2020. Immunity. 52(5):825-841. PubMed
  34. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  35. Barili V, et al. 2020. Nat Commun. 0.877777778. PubMed
  36. Dehmani S, et al. 2021. Front Immunol. 12:732530. PubMed
  37. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
  38. Volpin V, et al. 2020. Cancer Immunol Res. 8:1163. PubMed
  39. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  40. Lederer K, et al. 2022. Cell. . PubMed
  41. Chen Y, et al. 2020. Cell. 1496:183. PubMed
  42. Brudno JN, et al. 2020. Nat Med. 270:26. PubMed
  43. Chellappa S, et al. 2019. J Immunol. 202:1397. PubMed
  44. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  45. Rnjak D, et al. 2021. Transfus Clin Biol. . PubMed
  46. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  47. Diamantopoulos PT, et al. 2022. Cancers (Basel). 14:. PubMed
  48. Beyer M, et al. 2016. Nat Immunol. 17:593-603. PubMed
  49. Hu JQ, et al. 2020. J Cancer. 3.409722222. PubMed
  50. Tang X, et al. 2020. Sci Adv. 6:eaaz0374. PubMed
RRID
AB_2159324 (BioLegend Cat. No. 329917)
AB_2159324 (BioLegend Cat. No. 329918)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells

Ligand/Receptor
B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Other Formats

View All CD279 Reagents Request Custom Conjugation
Description Clone Applications
Brilliant Violet 421™ anti-human CD279 (PD-1) EH12.2H7 FC
Purified anti-human CD279 (PD-1) EH12.2H7 FC,Block,IHC-F
FITC anti-human CD279 (PD-1) EH12.2H7 FC
PE anti-human CD279 (PD-1) EH12.2H7 FC,SB
APC anti-human CD279 (PD-1) EH12.2H7 FC
Alexa Fluor® 647 anti-human CD279 (PD-1) EH12.2H7 FC
PerCP/Cyanine5.5 anti-human CD279 (PD-1) EH12.2H7 FC
APC/Cyanine7 anti-human CD279 (PD-1) EH12.2H7 FC
Pacific Blue™ anti-human CD279 (PD-1) EH12.2H7 FC
PE/Cyanine7 anti-human CD279 (PD-1) EH12.2H7 FC
Purified anti-human CD279 (PD-1) (Maxpar® Ready) EH12.2H7 FC,CyTOF®
Brilliant Violet 605™ anti-human CD279 (PD-1) EH12.2H7 FC
Ultra-LEAF™ Purified anti-human CD279 (PD-1) EH12.2H7 FC,Block,IHC-F
Brilliant Violet 711™ anti-human CD279 (PD-1) EH12.2H7 FC
Brilliant Violet 785™ anti-human CD279 (PD-1) EH12.2H7 FC
Brilliant Violet 510™ anti-human CD279 (PD-1) EH12.2H7 FC
Biotin anti-human CD279 (PD-1) EH12.2H7 FC
PE/Dazzle™ 594 anti-human CD279 (PD-1) EH12.2H7 FC
Alexa Fluor® 488 anti-human CD279 (PD-1) EH12.2H7 FC
PerCP anti-human CD279 (PD-1) EH12.2H7 FC
GoInVivo™ Purified anti-human CD279 (PD-1) EH12.2H7 FC,Block,IHC
Brilliant Violet 650™ anti-human CD279 (PD-1) EH12.2H7 FC
Alexa Fluor® 700 anti-human CD279 (PD-1) EH12.2H7 FC
APC/Fire™ 750 anti-human CD279 (PD-1) EH12.2H7 FC
TotalSeq™-A0088 anti-human CD279 (PD-1) EH12.2H7 PG
TotalSeq™-B0088 anti-human CD279 (PD-1) EH12.2H7 PG
TotalSeq™-C0088 anti-human CD279 (PD-1) EH12.2H7 PG
Brilliant Violet 750™ anti-human CD279 (PD-1) EH12.2H7 FC
TotalSeq™-D0088 anti-human CD279 (PD-1) EH12.2H7 PG
PE/Fire™ 640 anti-human CD279 (PD-1) EH12.2H7 FC
PE/Cyanine5 anti-human CD279 (PD-1) EH12.2H7 FC
PE/Fire™ 744 anti-human CD279 (PD-1) EH12.2H7 FC
Spark Red™ 718 anti-human CD279 (PD-1) EH12.2H7 FC
Brilliant Violet 570™ anti-human CD279 (PD-1) EH12.2H7 FC
Go To Top Version: 2    Revision Date: 01/22/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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