APC anti-human IgM Antibody anti-IgM

Clone: MHM-88 (See other available formats)
Regulatory Status: RUO
Other Names: Immunoglobulin M
Isotype: Mouse IgG1, κ
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Human peripheral blood lymphocytes stained with MHM-88 APC

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Cat #	Size	Price	Save
314509	25 tests	¥30,140
314510	100 tests	¥64,020

Description

IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Ig cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: MHM-88 antibody reacts with both soluble and membrane human immunoglobulin M (IgM). It does not react with other Ig isotypes. Additional reported applications (for the relevant formats) include: use as a primary or secondary reagent for ELISA analysis.

Due to the presence of excess soluble IgM in whole blood, which competes for antibody binding, staining for IgM on cells in whole blood is not recommended.
RRID: AB_493011 (BioLegend Cat. No. 314509)
AB_493011 (BioLegend Cat. No. 314510)

Antigen Details

Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
Cell Type: B cells
Biology Area: Immunology
Gene ID: 3507 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: IgM
Specificity Alt (DOES NOT SHOW ON TDS):: IgM
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about IgM on UniProt.org

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Other Formats

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Description	Clone	Applications
Purified anti-human IgM	MHM-88	FC,ELISA
PE anti-human IgM	MHM-88	FC
Biotin anti-human IgM	MHM-88	FC,ELISA
FITC anti-human IgM	MHM-88	FC
APC anti-human IgM	MHM-88	FC
PerCP/Cyanine5.5 anti-human IgM	MHM-88	FC
Pacific Blue™ anti-human IgM	MHM-88	FC
Brilliant Violet 421™ anti-human IgM	MHM-88	FC
APC/Cyanine7 anti-human IgM	MHM-88	FC
Brilliant Violet 570™ anti-human IgM	MHM-88	FC
Brilliant Violet 510™ anti-human IgM	MHM-88	FC
Brilliant Violet 605™ anti-human IgM	MHM-88	FC
Brilliant Violet 650™ anti-human IgM	MHM-88	FC
Purified anti-human IgM (Maxpar® Ready)	MHM-88	FC,CyTOF®
PE/Dazzle™ 594 anti-human IgM	MHM-88	FC
PE/Cyanine7 anti-human IgM	MHM-88	FC
Alexa Fluor® 488 anti-human IgM	MHM-88	FC
Alexa Fluor® 647 anti-human IgM	MHM-88	FC
Alexa Fluor® 700 anti-human IgM	MHM-88	FC
Brilliant Violet 711™ anti-human IgM	MHM-88	FC
TotalSeq™-A0136 anti-human IgM	MHM-88	PG
TotalSeq™-C0136 anti-human IgM	MHM-88	PG
Brilliant Violet 785™ anti-human IgM	MHM-88	FC
APC/Fire™ 750 anti-human IgM	MHM-88	FC
TotalSeq™-B0136 anti-human IgM	MHM-88	PG
Spark Violet™ 423 anti-human IgM Antibody	MHM-88	FC
TotalSeq™-D0136 anti-human IgM	MHM-88	PG
Spark Blue™ 550 anti-human IgM	MHM-88	FC
Spark Blue™ 515 anti-human IgM	MHM-88	FC
PE/Fire™ 700 anti-human IgM	MHM-88	FC
Spark YG™ 593 anti-human IgM	MHM-88	FC
PE/Fire™ 640 anti-human IgM	MHM-88	FC
Spark Red™ 718 anti-human IgM (Flexi-Fluor™)	MHM-88	FC
APC anti-human IgM	MHM-88	FC
PE anti-human IgM	MHM-88	FC
Brilliant Violet 750™ anti-human IgM	MHM-88	FC

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Go To Top Version: 3 Revision Date: 10/10/2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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