Loading...

APC/Cyanine7 anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104_APCCyanine7_CD45dot2_Antibody_013019_updated.png
C57BL/6 mouse splenocytes were stained with CD45.2 (clone 104) APC/Cyanine7 (filled histogram) or Mouse IgG2a, κ APC/Cyanine7 isotype control (open histogram).
  • 104_APCCyanine7_CD45dot2_Antibody_013019_updated.png
    C57BL/6 mouse splenocytes were stained with CD45.2 (clone 104) APC/Cyanine7 (filled histogram) or Mouse IgG2a, κ APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Save
109823 25 µg ¥25,520
109824 100 µg ¥64,240
Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  2. Yamazaki S, et al. 2022. Mucosal Immunol. :. PubMed
  3. Leung W, et al. 2022. Cancer Discov. 12:1782. PubMed
  4. Shen Z, et al. 2022. EBioMedicine. 85:104285. PubMed
  5. Yoo HJ, et al. 2022. Cell Rep. 41:111598. PubMed
  6. Pleuger C, et al. 2022. Elife. 11: . PubMed
  7. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  8. Kasuya T, et al. 2023. Sci Rep. 13:1653. PubMed
  9. Cheng H, et al. 2023. Nat Metab. 5:314. PubMed
  10. Ernst MPT, et al. 2023. Hemasphere. 7:e824. PubMed
  11. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  12. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  13. Chang D, et al. 2020. Immunity. 53(3):614-626.e4. PubMed
  14. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  15. Xu H, et al. 2023. Elife. 12:. PubMed
  16. Chang D, et al. 2023. Front Immunol. 14:1105145. PubMed
  17. Zhang AQ, et al. 2023. Nat Biomed Eng. . PubMed
  18. Gravano D, et al. 2010. PLoS One. 5:e13528. PubMed
  19. Huang J, et al. 2021. Immunity. 54(4):673-686.e4. PubMed
  20. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  21. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
  22. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  23. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  24. Adrover JM, et al. 2022. JCI Insight. 7:. PubMed
  25. Drajac C, et al. 2021. Mucosal Immunol. 14:949. PubMed
  26. Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed
  27. Iturri L, et al. 2021. Immunity. . PubMed
  28. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  29. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  30. Tran I, et al. 2013. J Clin Invest. 123:1590. PubMed
  31. Kang S, et al. 2011. J Exp Med. 208:747. PubMed
  32. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  33. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  34. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  35. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  36. Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
  37. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  38. LaFleur MW, et al. 2019. Nat Immunol. 20:1335. PubMed
  39. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  40. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  41. Marangoni F, et al. 2021. Cell. . PubMed
  42. Xu M et al. 2018. Immunity. 48(4):787-798 . PubMed
  43. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  44. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  45. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  46. Jeong SH, et al. 2021. Nat Commun. 12:4405. PubMed
  47. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  48. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  49. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  50. Schepanski S, et al. 2022. Nat Commun. 13:4571. PubMed
  51. Li J, et al. 2020. Elife. 9:00. PubMed
  52. Yang Z, et al. 2020. Sci Rep. 17757:10. PubMed
  53. Thiriot A et al. 2017. BMC biology. 15(1):45 . PubMed
  54. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  55. Codina A, et al. 2019. Cell Syst. 8:136. PubMed
  56. Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed
  57. Wang W, et al. 2020. Immunity. 51(6):1102-1118.e7.. PubMed
  58. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  59. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  60. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  61. Li H, et al. 2011. Blood. 117:697. PubMed
  62. Béguelin W, et al. 2020. Cancer Cell. 37(5):655-673. PubMed
  63. White C, et al. 2015. J Immunol. 194:697. PubMed
  64. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  65. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  66. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  67. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  68. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  69. Chen F, et al. 2022. Cell Rep. 38:110215. PubMed
  70. Ishikura S, et al. 2021. J Cell Biochem. . PubMed
  71. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  72. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  73. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  74. Li C, et al. 2018. Cell. 174:285. PubMed
  75. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  76. Wang X, et al. 2021. Cell. 184:5357. PubMed
  77. Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed
  78. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
  79. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  80. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  81. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  82. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  83. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  84. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  85. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  86. Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed
  87. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  88. Glodde N et al. 2017. Immunity. 47(4):789-802 . PubMed
  89. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  90. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  91. Barcia Durán JG, et al. 2021. Commun Biol. 4:406. PubMed
  92. Zheng Y, et al. 2022. Transl Res. :. PubMed
  93. Bretz N, et al. 2014. Immunol Lett. 161:140. PubMed
  94. Peloquin GL, et al. 2019. Respir Res. 20:123. PubMed
  95. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
  96. Doo DW, et al. 2020. Ther Adv Med Oncol. 12:1758835920913798. PubMed
  97. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  98. Song W, et al. 2022. Immunity. 55:290. PubMed
  99. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
  100. Effern M, et al. 2020. Immunity. 53(3):564-580.e9. PubMed
  101. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
RRID
AB_830788 (BioLegend Cat. No. 109823)
AB_830788 (BioLegend Cat. No. 109824)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD45.2
Specificity Alt (DOES NOT SHOW ON TDS):
CD45.2
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD45.2 on UniProt.org

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Go To Top Version: 2    Revision Date: 01/30/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account