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LEAF™ Purified anti-mouse CD28 Antibody

Pricing & Availability
Clone
37.51 (See other available formats)
Regulatory Status
RUO
Other Names
Tp44, T44
Isotype
Syrian Hamster IgG
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Product Citations
publications
37pt51_LEAF_081908.jpg
C57BL/6 splenocytes stained with LEAF™ purified 37.51, followed by anti-Syrian hamster IgG FITC
  • 37pt51_LEAF_081908.jpg
    C57BL/6 splenocytes stained with LEAF™ purified 37.51, followed by anti-Syrian hamster IgG FITC
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This product has been discontinued as a part of our ongoing LEAF™ to Ultra-LEAF™ antibody transition.
The equivalent Ultra-LEAF™ product may be available at comparable or significantly lower price.

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Description

CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Syrian Hamster
Immunogen
C57BL/6 mouse T-cell lymphoma EL-4
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.1 EU/µg of the protein (<0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The CD28 antibody solution should be stored undiluted between 2°C and 8°C. This LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
IP, IHC-F, Costim, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.


This product is included in our Activation Bundles. Learn more...
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).

Application References

(PubMed link indicates BioLegend citation)
  1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
  2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
  3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
  4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
  5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
  6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
  8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
  9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
  10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
  11. Barbi J, et al. 2007. Blood 110:2215.
  12. Milpied P, et al. 2011. Blood 118:2993. PubMed
  13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
  14. Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
  15. Li CR, et al. 2014. J Immunol. 192:1425. PubMed
  16. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
Product Citations
  1. Logan K Smith et al. 2018. Immunity. 48(2):299-312 . PubMed
  2. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  3. Aki D, et al. 2018. Nat Immunol. 19:p766. PubMed
  4. Jayachandran R, et al. 2019. Immunity. 50:152. PubMed
  5. Du C, et al. 2016. Nat Commun. 7: 11120. PubMed
  6. Page N, et al. 2018. Immunity. 48:937. PubMed
  7. Duong–Ly KC, et al. 2018. J Cell Sci. 131:17. PubMed
  8. Liu B, et al. 2019. Nat Commun. 10:354. PubMed
  9. Li J, et al. 2019. Theranostics. 9:4324. PubMed
  10. Sanin DE et al. 2018. Immunity. 49(6):1021-1033 . PubMed
  11. Klein Geltink RI et al. 2017. Cell. 171(2):385-397 . PubMed
  12. Magod P, et al. 2021. Cell Reports. 36(5):109480. PubMed
  13. Hawila E, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.10.104. PubMed
  14. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  15. Ren W, et al. 2018. Mucosal Immunol. 12:531. PubMed
  16. Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
  17. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  18. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  19. Alessandro Torgovnick et al. 2018. Cell reports. 25(4):1027-1039 . PubMed
  20. De Cicco P, et al. 2019. British Journal of Pharmacology. 177(4):884-897. PubMed
  21. Chen H, et al. 2009. Prostaglandins Leukot Essent Fatty Acids. 80:195. PubMed
  22. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  23. Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed
  24. Kimmerling R, et al. 2016. Nat Commun. 7:10220. PubMed
  25. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  26. Rybakin V, Gascoigne N 2012. PLoS One. 7:e43191. PubMed
  27. Myers R, et al. 2013. J Immunol. 190:6287. PubMed
  28. Katsuyama T, et al. 2021. Cell Reports. 36(1):109339. PubMed
  29. Nagashima H, et al. 2016. J Immunol. 196: 4082 - 4089. PubMed
  30. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed

Antigen Details

Structure
Ig superfamily, 44 kD
Distribution

Thymocytes, CD4+, CD8+ peripheral T cells, NK cells

Function
Costimulates T and NK cells
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
NK cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.

Gene ID
12487 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD28
Specificity Alt (DOES NOT SHOW ON TDS):
CD28
App Abbreviation (DOES NOT SHOW ON TDS):
FC, IP, IHC-F, Costim, Block
UniProt
View information about CD28 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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