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A single cell suspension from human PBMCs was prepared to isolate hematopoietic progenitor cells using the MojoSort™ Human Hematopoietic Progenitor Cell Isolation Kit. Cells were stained with CD34 (clone 581) PE and CD45 (clone 2D1) APC. Dead cells were excluded by 7-AAD. -
| Cat # | Size | Price | Save |
|---|---|---|---|
| 480095 | 20 tests | ¥48,180 | |
| 480096 | 200 tests | ¥143,880 |
Human non hematopoietic progenitor cells are depleted by incubating the sample with the Human Hematopoietic Progenitor Cell Isolation Biotin Antibody Cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched human hematopoietic progenitor cells are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat. No. 480095:
- 200 µl Biotin-Antibody Cocktail
- 200 µl Streptavidin Nanobeads
For Cat. No. 480096:
- 2 vials of 1 ml Biotin-Antibody Cocktail each
- 2 vials of 1 ml Streptavidin Nanobeads each
Product Details
- Verified Reactivity
- Human
- Formulation
-
Cocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide. - Preparation
-
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads. - Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
-
10 µl of antibody cocktail for 1 X 107 cells in 100 µl of buffer.
10 µl Streptavidin Nanobeads for 1 X 107 cells in 100 µl of buffer.
- Application Notes
-
This kit is designed for the isolation of untouched Human Hematopoietic Progenitor Cell from peripheral blood mononuclear cells (PBMCs).
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 5X
Nanobead dilution to use in columns: 15X
Antigen Details
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
- Molecular Family
- CD Molecules
- Gene ID
- NA
- App Abbreviation (DOES NOT SHOW ON TDS):
- Cell Sep - Neg
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
-
MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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