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A single cell suspension from C57BL/6 mouse splenocytes was prepared for positive selection of CD3+ cells using the MojoSort™ Mouse CD3 selection Kit. Cells were stained with anti-mouse CD3 (clone 145-2C11) PE. Dead cells was excluded by 7-AAD. -
| Cat # | Size | Price | Save |
|---|---|---|---|
| 480099 | 20 tests | ¥43,560 | |
| 480100 | 200 tests | ¥130,680 |
Mouse CD3+ cells are either selected or depleted by incubating the sample with the biotin conjugated anti-mouse CD3 antibody followed by Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. After collection of the CD3+ expressing cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc. Clone 17A2 recognizes ε/γ (but not ε/δ) of the CD3 complex. It can induce T cell activation.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat. No. 480099:
- 1 vial containing 200 µl of Biotin anti-mouse CD3 antibody (clone 17A2)
- 1 vial containing 200 µl Streptavidin Nanobeads
For Cat. No. 480100:
- 2 vials containing 1ml each of Biotin anti-mouse CD3 antibody (clone 17A2)
- 2 vials containing 1ml each of Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Mouse
- Formulation
-
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, pH 7.2 with stabilizer.
Streptavidin Nanobeads: Aqueous solution containing 0.05% sodium azide and 0.3% BSA. - Preparation
-
The antibody was purified by affinity chromatography, and conjugated wtih biotin under optimal conditions.
Streptavidin Nanobeads: protein-coated magnetic nanobeads. - Storage & Handling
- Antibody cocktail and Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
-
10 µl of Biotin anti-mouse CD3 antibody for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer. - Application Notes
-
This kit is designed for the positive selection or depletion of mouse CD3+ cells from lymphoid tissue.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 8X
Nanobead dilution to use in columns: 4X - Product Citations
-
Antigen Details
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, TCRs
- Gene ID
- NA
- Specificity (DOES NOT SHOW ON TDS):
- CD3
- App Abbreviation (DOES NOT SHOW ON TDS):
- Cell Sep - Pos
- UniProt
- View information about CD3 on UniProt.org
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
-
MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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