Loading...

PE anti-mouse CD45.2 Antibody

Pricing & Availability
Clone
104 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-5.2, LCA
Isotype
Mouse (SJL) IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
104
BALB/c (solid line) and SJL (broken line) splenocytes stained with 104 PE
  • 104
    BALB/c (solid line) and SJL (broken line) splenocytes stained with 104 PE
Compare all formats See PE spectral data
Cat # Size Price Save
109807 50 µg ¥13,420
109808 200 µg ¥47,080
Description

CD45.2 is an alloantigen of CD45, expressed by Ly5.2 bearing mouse strains (e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in the mouse that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
B10.S mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 104 antibody does not react with mouse cells expressing the CD45.1 alloantigen. Additional reported applications (for the relevant formats) include: immunoprecipitation4, in vivo and in vitro blocking of B cell responses1,2, and immunohistochemical staining of acetone-fixed frozen sections3. 

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Suzuki K, et al. 2000. Immunity 13:691. (IHC)
  4. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  5. Baldwin TA and Hogquist KA. 2007. J. Immunol. 179:837.
  6. Pascal V, et al. 2007. J. Immunol. 179:1751.
  7. Burman AC, et al. 2007. Blood 110:1064.
  8. Kincaid EZ, et al. 2007. J. Immunol. 179:3187.
  9. Phan TG, et al. 2007. Nature Immunol. 8:992.
  10. Nakano-Yokomizo T, et al. 2011. J. Exp Med. 208:1661. PubMed
  11. Wen T, et al. 2013. PNAS. 110:6067. PubMed
  12. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Takihara Y, et al. 2022. Commun Biol. 5:776. PubMed
  2. Lehmkuhl P, et al. 2021. Cell Mol Immunol. 18:1677. PubMed
  3. Ozga AJ, et al. 2022. Immunity. 55:82. PubMed
  4. He D, et al. 2022. Immunity. 55:159. PubMed
  5. Wang H, et al. 2022. Immunol Cell Biol. 100:547. PubMed
  6. Nozaki K, et al. 2022. Nature. 606:960. PubMed
  7. Shemesh A, et al. 2022. J Exp Med. 219: . PubMed
  8. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  9. Nishina T, et al. 2023. iScience. 26:105934. PubMed
  10. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  11. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  12. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  13. Tas JMJ, et al. 2022. Immunity. 55:1856. PubMed
  14. Melzi E, et al. 2022. Immunity. 55:2168. PubMed
  15. Yi C, et al. 2023. J Neuroinflammation. 20:45. PubMed
  16. Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed
  17. Oh J, et al. 2023. Nat Commun. 14:3278. PubMed
  18. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  19. Lee S, et al. 2021. JCI Insight. 6:. PubMed
  20. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  21. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  22. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  23. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  24. Bonnet C, et al. 2021. iScience. 24:103399. PubMed
  25. Pack AD, et al. 2021. Cell Reports. 36:109586. PubMed
  26. Hirano KI, et al. 2021. eLife. 0.416666666666667. PubMed
  27. Koon H, et al. 2012. J Vis Exp. 68: 4208. PubMed
  28. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  29. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  30. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  31. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  32. Davila S, et al. 2014. J Immunol. 192:4284. PubMed
  33. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  34. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  35. Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
  36. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  37. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  38. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  39. Amor C, et al. 2020. Nature. 583:127. PubMed
  40. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  41. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  42. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  43. Rengarajan S, et al. 2020. Cell Rep Med. :1. PubMed
  44. He L, et al. 2019. iScience. 0.790972222. PubMed
  45. Kaplan BLF et al. 2018. Current protocols in toxicology. 75:11:00 25. PubMed
  46. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  47. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  48. Russler-Germain EV, et al. 2021. Elife. 10:. PubMed
  49. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  50. Chen D, et al. 2021. Molecular Cell. 81:3833. PubMed
  51. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  52. Mansell E, et al. 2020. Cell Stem Cell. . PubMed
  53. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  54. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  55. Horiguchi H, et al. 2019. Genes Dev. 33:1641. PubMed
  56. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  57. Ndeupen S, et al. 2022. STAR Protoc. 3:101350. PubMed
  58. Zhang X, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  59. Tang Q, et al. 2021. Immunity. 54:1961. PubMed
  60. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  61. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  62. Wang X, et al. 2021. EMBO J. 40:e105926. PubMed
  63. Hirata T, et al. 2012. Int Immunol. 24:705. PubMed
  64. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  65. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  66. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  67. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  68. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  69. Xiong J, et al. 2011. Int Immunol. 23:56. PubMed
  70. Wang X, et al. 2021. Cell. 184:5357. PubMed
  71. Koide S, et al. 2022. iScience. 25:103603. PubMed
  72. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  73. Contreras NA, et al. 2019. PLoS Pathog. 15:e1007890. PubMed
  74. Sun Y, et al. 2020. J Immunol. 205:2649. PubMed
  75. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  76. Wang X, et al. 2022. Elife. 11:. PubMed
  77. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  78. Sindrilaru A, et al. 2009. Blood. 113:5266. PubMed
  79. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  80. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  81. Spath S, et al. 2022. iScience. 25:104998. PubMed
  82. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  83. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  84. Huang X, et al. 2021. Immunity. . PubMed
  85. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
  86. Kaw S, et al. 2020. EMBO J. 39:e105594. PubMed
  87. Liu Y, et al. 2021. Nat Commun. 12:6831. PubMed
  88. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
  89. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  90. Young K, et al. 2021. Cell Stem Cell. . PubMed
  91. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  92. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  93. Finkin S et al. 2019. Immunity. 51(2):324-336 . PubMed
  94. Huang LH et al. 2018. Cell metabolism. 29(2):475-487 . PubMed
RRID
AB_313444 (BioLegend Cat. No. 109807)
AB_313444 (BioLegend Cat. No. 109808)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.2 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Suzuki K, et al. 2000. Immunity 13:691.

Gene ID
19264 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD45.2
Specificity Alt (DOES NOT SHOW ON TDS):
CD45.2
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD45.2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD45.2 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse CD45.2 104 FC
FITC anti-mouse CD45.2 104 FC
PE anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 104 FC,Block,IHC-F,IP
APC anti-mouse CD45.2 104 FC
Alexa Fluor® 488 anti-mouse CD45.2 104 FC
Alexa Fluor® 647 anti-mouse CD45.2 104 FC
Pacific Blue™ anti-mouse CD45.2 104 FC
Alexa Fluor® 700 anti-mouse CD45.2 104 FC
APC/Cyanine7 anti-mouse CD45.2 104 FC
PerCP anti-mouse CD45.2 104 FC
PerCP/Cyanine5.5 anti-mouse CD45.2 104 FC
PE/Cyanine7 anti-mouse CD45.2 104 FC
Brilliant Violet 421™ anti-mouse CD45.2 104 FC
Brilliant Violet 570™ anti-mouse CD45.2 104 FC
Brilliant Violet 650™ anti-mouse CD45.2 104 FC
Brilliant Violet 510™ anti-mouse CD45.2 104 FC
Brilliant Violet 785™ anti-mouse CD45.2 104 FC
Brilliant Violet 605™ anti-mouse CD45.2 104 FC
Purified anti-mouse CD45.2 (Maxpar® Ready) 104 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD45.2 104 FC
Brilliant Violet 711™ anti-mouse CD45.2 104 FC
Alexa Fluor® 594 anti-mouse CD45.2 104 IHC-F,SB
APC/Fire™ 750 anti-mouse CD45.2 104 FC
TotalSeq™-A0157 anti-mouse CD45.2 104 PG
TotalSeq™-B0157 anti-mouse CD45.2 104 PG
TotalSeq™-C0157 anti-mouse CD45.2 104 PG
Brilliant Violet 750™ anti-mouse CD45.2 104 FC
Spark Blue™ 550 anti-mouse CD45.2 104 FC
Spark NIR™ 685 anti-mouse CD45.2 104 FC
Spark UV™ 387 anti-mouse CD45.2 104 FC
Spark Blue™ 574 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Spark Red™ 718 anti-mouse CD45.2 (Flexi-Fluor™) 104 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account