PE/Fire™ 640 anti-human CD279 (PD-1) antibody, CD279, EH12.2H7

Clone: EH12.2H7 (See other available formats)
Regulatory Status: RUO
Other Names: PD-1, PDCD1
Isotype: Mouse IgG1, κ
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Human peripheral blood lymphocytes were stained with anti-human CD3 FITC and anti-human CD279 (PD-1) PE/Fire™ 640 (clone EH12.2H7) (left), or mouse IgG1, κ PE/Fire™ 640 (right).

Compare all formats See PE/Fire™ 640 spectral data

Cat #	Size	Price	Save
329967	25 tests	¥42,680
329968	100 tests	¥91,740

Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: blocking of ligand binding^1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections¹³, and spatial biology (IBEX)^15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
RRID: AB_2894474 (BioLegend Cat. No. 329967)
AB_2894474 (BioLegend Cat. No. 329968)

Antigen Details

Structure: Immunoglobulin superfamily
Distribution: Transiently expressed on CD4^- CD8^- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells
Ligand/Receptor: B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type: B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Gene ID: 5133 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: CD279
Specificity Alt (DOES NOT SHOW ON TDS):: CD279
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about CD279 on UniProt.org

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Other Formats

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Description	Clone	Applications
Brilliant Violet 421™ anti-human CD279 (PD-1)	EH12.2H7	FC
Purified anti-human CD279 (PD-1)	EH12.2H7	FC,Block,IHC-F
FITC anti-human CD279 (PD-1)	EH12.2H7	FC
PE anti-human CD279 (PD-1)	EH12.2H7	FC,SB
APC anti-human CD279 (PD-1)	EH12.2H7	FC
Alexa Fluor® 647 anti-human CD279 (PD-1)	EH12.2H7	FC
PerCP/Cyanine5.5 anti-human CD279 (PD-1)	EH12.2H7	FC
APC/Cyanine7 anti-human CD279 (PD-1)	EH12.2H7	FC
Pacific Blue™ anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Cyanine7 anti-human CD279 (PD-1)	EH12.2H7	FC
Purified anti-human CD279 (PD-1) (Maxpar® Ready)	EH12.2H7	FC,CyTOF®
Brilliant Violet 605™ anti-human CD279 (PD-1)	EH12.2H7	FC
Ultra-LEAF™ Purified anti-human CD279 (PD-1)	EH12.2H7	FC,Block,IHC-F
Brilliant Violet 711™ anti-human CD279 (PD-1)	EH12.2H7	FC
Brilliant Violet 785™ anti-human CD279 (PD-1)	EH12.2H7	FC
Brilliant Violet 510™ anti-human CD279 (PD-1)	EH12.2H7	FC
Biotin anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Dazzle™ 594 anti-human CD279 (PD-1)	EH12.2H7	FC
Alexa Fluor® 488 anti-human CD279 (PD-1)	EH12.2H7	FC
PerCP anti-human CD279 (PD-1)	EH12.2H7	FC
GoInVivo™ Purified anti-human CD279 (PD-1)	EH12.2H7	FC,Block,IHC
Brilliant Violet 650™ anti-human CD279 (PD-1)	EH12.2H7	FC
Alexa Fluor® 700 anti-human CD279 (PD-1)	EH12.2H7	FC
APC/Fire™ 750 anti-human CD279 (PD-1)	EH12.2H7	FC
TotalSeq™-A0088 anti-human CD279 (PD-1)	EH12.2H7	PG
TotalSeq™-B0088 anti-human CD279 (PD-1)	EH12.2H7	PG
TotalSeq™-C0088 anti-human CD279 (PD-1)	EH12.2H7	PG
Brilliant Violet 750™ anti-human CD279 (PD-1)	EH12.2H7	FC
TotalSeq™-D0088 anti-human CD279 (PD-1)	EH12.2H7	PG
PE/Fire™ 640 anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Cyanine5 anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Fire™ 744 anti-human CD279 (PD-1)	EH12.2H7	FC
Spark Red™ 718 anti-human CD279 (PD-1)	EH12.2H7	FC
Brilliant Violet 570™ anti-human CD279 (PD-1)	EH12.2H7	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 08/17/2021

For Research Use Only. Not for diagnostic or therapeutic use.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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