Loading...

Purified anti-Neurofilament Marker (pan axonal, cocktail) Antibody

Pricing & Availability
Clone
SMI 312 (See other available formats)
Regulatory Status
RUO
Other Names
SMI-312, SMI312
Isotype
Mouse IgG1/Mouse IgM
Ave. Rating
Submit a Review
Product Citations
publications
A_SMI312_PURE_NFMarker_CktlAb_IHCP_RatOrig_011816
IHC staining of purified anti-Neurofilament Marker (pan axonal, cocktail) antibody (clone SMI 312) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • A_SMI312_PURE_NFMarker_CktlAb_IHCP_RatOrig_011816
    IHC staining of purified anti-Neurofilament Marker (pan axonal, cocktail) antibody (clone SMI 312) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • B_SMI312_PURE_NFMarker_CktlAb_IHCP_Mu_011918
    IHC staining of purified anti-Neurofilament Marker (pan axonal, cocktail) antibody (clone SMI 312) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • C_SMI312_PURE_NFMarker_CktlAb_IHCP_Rat_011918
    IHC staining of purified anti-Neurofilament Marker (pan axonal, cocktail) antibody (clone SMI 312) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Retrieval-ALL Antigen Unmasking System 3 (Cat. No. 927601), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • D_SMI312_PURE_NFMarker_CktlAb_WB_011918
    Western blot of purified anti-Neurofilament Marker (pan axonal, cocktail) antibody (clone SMI 312). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of Mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 ug/mL of clone SMI 312 or mouse IgG1 overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-Tubulin Beta 3 (TUBB3) antibody (clone AA10, Cat. No. 657409) was used as a loading control. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Save
837904 100 µg ¥60,720
Description

Neurofilaments (NF) are approximately 10 nanometer intermediate filaments found in neurons. They are a major component of the neuronal cytoskeleton and their function is primarily to provide structural support for the axon and to regulate axon diameter. There are three major NF subunits, and the names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE. The light or lowest (NF-L) runs at 68-70 kD, the medium or middle (NF-M) runs at about 145-160 kD, and the heavy or highest (NF-H) runs at 200-220 kD. However, the actual molecular weight of these proteins is considerably lower due to the highly charged C-terminal regions of the molecules. The level of NF gene expression correlates with the axonal diameter, which controls how fast electrical signals travel down the axon. Mutant mice with NF abnormalities have phenotypes resembling amyotrophic lateral sclerosis. NF immunostaining is common in diagnostic neuropathology. It is useful for differentiating neurons (positive for NF) from glia (negative for NF).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Homogenized hypothalami recovered from Fischer 344 rats.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
EM, ICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry1, 6, 12, 16, 19, immunofluorescent staining3, 8, 9, 18.

SMI 312 is a mixture of monoclonal antibodies that react against complex networks of axons. It is directed against extensively phosphorylated axonal epitopes on neurofilaments M and H. SMI 312 has been selected to provide a specific marker for axons in tissue sections and cultures. In contrast to individual anti-phosphoneurofilament antibodies that identify different subsets of neurofilament phosphoepitopes, which are suitable for defining functional and regional differences in normal and pathologic axons, SMI 312 is a convenient marker for axons in general. SMI 312 visualizes axons in an area-specific maturation pattern in human fetal brain. The antibody cocktail defines nuclear borderlines and is useful in establishing early connectivity with SMI 311, anti-neurofilament (not phosphorylated) identified dendrites. SMI 312 visualizes aberrantly sprouting axons in neuritic plaques derived from cortico-cortical fibers in Alzheimer's disease and identifies loss of synaptic circuitry proposed to be the basis of memory.

Application References

(PubMed link indicates BioLegend citation)
  1. Sternberger LA, et al. 1982. Proc. Natl. Acad. Sci. USA. 79:1326. (IHC, ICC)
  2. Choi Y, et al. 2008. Genes & Dev. 22:2485. (IHC) PubMed
  3. BussiFre T, et al. 2004. Am. J. Pathol. 165:987. (IHC-F)
  4. Chung RS, et al. 2003. J. Neurosci. 23:3336. (IHC)
  5. De Repentigny Y, et al. 2011. PLoS One. 6:e21093. (IHC)
  6. Wilkins A, et al. 2003. J. Neurosci. 23:4967. (ICC)
  7. Rudinskiy N, et al. 2012. Nat. Neurosci. 15:1422. (IHC)
  8. Wang JY, et al. 2014. Dev. Cell. 28:670. (EM)
  9. Canetta SE, et al. 2011. PLoS One. 6:e25108. (ICC) PubMed
  10. Nicaise C, et al. 2012. J. Neurotrauma. 29:2748. (IHC)
  11. Ma M, et al. 2013. Neurobiol. Dis. 56:34. (IHC)
  12. Zurashvili T, et al. 2013. Mol. Cell Biol. 33:1027. (ICC)
  13. Powers BE, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:4075. (IHC)
  14. Riddle A, et al. 2012. Stroke. 43:178. (IHC)
  15. Sahni V, et al. 2010. Neurosci. 30:1839. (IHC)
  16. Liu HY, et al. 2013. J. Neurosci. 33:11479. (ICC)
  17. Nicaise C, et al. 2013. J. Neurotrauma. 30:1092. (IHC)
  18. Klusch A, et al. 2013. J. Invest. Dermatol. 133:1387. (ICC) PubMed
  19. Huang TN, et al. 2014. Nat. Neurosci. 17:240. (ICC)
Product Citations
  1. Shah B, et al. 2016. Cereb Cortex. 10.1093/cercor/bhv341. PubMed
  2. Matsumura R, et al. 2018. Sci Rep. 9:797. PubMed
  3. Scaricamazza S, et al. 2022. Br J Pharmacol. 179:1732. PubMed
  4. Silverå Ejneby M, et al. 2022. Nat Biomed Eng. 6:741. PubMed
  5. Castoldi V, et al. 2022. J Neurosci Res. 100:855. PubMed
  6. Southam K, et al. 2022. J Physiol. 600:1611. PubMed
  7. Ackermans NL, et al. 2022. Acta Neuropathol. 144:5. PubMed
  8. Schwarzenberg FL, et al. 2022. Front Immunol. 13:947961. PubMed
  9. Su X, et al. 2023. Int J Mol Med. 51: . PubMed
  10. Junakovic A, et al. 2023. Brain Struct Funct. 228:613. PubMed
  11. Unda BK, et al. 2023. Mol Psychiatry. :. PubMed
  12. Mendonsa S, et al. 2023. Nat Neurosci. 26:394. PubMed
  13. Kapell H, et al. 2023. J Clin Invest. :. PubMed
  14. Prieto Huarcaya S, et al. 2022. Autophagy. 18:1127. PubMed
  15. Abedin MJ, et al. 2023. Front Bioeng Biotechnol. 11:1150772. PubMed
  16. Woelfle S, et al. 2023. BMC Biol. 21:113. PubMed
  17. So HK, et al. 2023. Research (Wash D C). 6:0158. PubMed
  18. Geerts C, et al. 2017. PLoS One. 10.1371/journal.pone.0180912. PubMed
  19. Eltahir MK, et al. 2022. PLoS One. 17:e0269683. PubMed
  20. Soto-Faguás CM, et al. 2021. Acta Neuropathol Commun. 9:162. PubMed
  21. Mehta AR, et al. 2021. Acta Neuropathol. 141:257. PubMed
  22. Claus L, et al. 2017. Cereb Cortex. 10.1093/cercor/bhw368. PubMed
  23. Weber R, et al. 2017. NMR Biomed. 10.1002/nbm.3717. PubMed
  24. Kao CS, et al. 2020. Nat Commun. 4.141666667. PubMed
  25. Song JM, et al. 2021. The Journal of Neuroscience. 41(11):2344-2359. PubMed
  26. Alada-Morais S, et al. 2021. Cerebral Cortex. :. PubMed
  27. Giandomenico SL, et al. 2019. Nat Neurosci. 22:669. PubMed
  28. Pons V, et al. 2021. Alzheimers Res Ther. 13:8. PubMed
  29. Kanaan NM, et al. 2021. Front Mol Neurosci. 14:607303. PubMed
  30. Haytural H, et al. 2021. Brain Commun. 3:fcab079. PubMed
  31. Tan C, et al. 2019. Neuron. 101:920. PubMed
  32. Plemel J,et al. 2017. Glia. . 10.1002/glia.23245. PubMed
  33. Lickfett S, et al. 2022. STAR Protoc. 3:101567. PubMed
  34. Doron-Mandel E, et al. 2021. EMBO J. 40:e107158. PubMed
  35. Zigler Jr. J, et al. 2016. PLoS One. 11: 0160447. PubMed
  36. Zappulo A, et al. 2017. Nat Commun. 10.1038/s41467-017-00690-6. PubMed
  37. Urban MW et al. 2019. J Neurotrauma. 37(3):572-579 . PubMed
  38. Qiang L, et al. 2019. Hum Mol Genet. 28:1136. PubMed
  39. Shao W, et al. 2020. Nature. 580:106. PubMed
  40. Shen Y, et al. 2020. Theranostics. 10:11794. PubMed
  41. Uyeda A, et al. 2021. Glia. 69:2591. PubMed
  42. Yamada S, et al. 2022. PLoS Genet. 18:e1010438. PubMed
  43. Zhu Q, et al. 2021. Cell Reports. 36(9):109639. PubMed
  44. So Hyun Kim, Sun-Kyoung Im 2017. Nat Commun. 8:14346. PubMed
  45. Zhu X, et al. 2020. J Neuroinflammation. 17:78. PubMed
  46. Li P, et al. 2022. Nat Commun. 13:3130. PubMed
  47. Yamada S, et al. 2021. iScience. 24:103484. PubMed
  48. Sundaramoorthy V, et al. 2020. PLoS Pathog. 16:e1008343. PubMed
  49. Zhang R, et al. 2021. Neurosci Bull. 37:1091. PubMed
  50. Creighton BA, et al. 2021. Elife. 10:. PubMed
  51. Yeh SI, et al. 2021. Invest Ophthalmol Vis Sci. 62:23:00. PubMed
  52. Huang YA, et al. 2020. J Cell Sci. 133:00:00. PubMed
  53. Schulz A, et al. 2016. PLoS One. 11: 0159718. PubMed
  54. Wu F, et al. 2019. J Neurosci. 39:7369. PubMed
  55. Hamilton AM, et al. 2019. Sci Rep. 9:8488. PubMed
  56. Cheng WH, et al. 2019. Alzheimers Res Ther. 11:6. PubMed
  57. Partida GJ et al. 2018. The Journal of Neuroscience. 38(37):8087-8105 . PubMed
  58. Hoffmann PC, et al. 2021. Elife. 10:. PubMed
  59. Jensen SK, et al. 2018. Cell Rep. 24:3167. PubMed
  60. Aiken J, et al. 2019. Hum Mol Genet. 28:1227. PubMed
  61. Fifield KE, et al. 2020. European Journal of Neuroscience. 52(4):3196-3214.. PubMed
  62. Huuskonen MT, et al. 2021. Front Immunol. 12:785519. PubMed
  63. Ehrlich M, et al. 2017. Proc Natl Acad Sci U S A. 114(11):E2243-E2252. PubMed
  64. Choi Y, et al. 2008. Genes Dev. 22:2485-2495. PubMed
  65. Iannielli A, et al. 2019. Cell Rep. 29:4646. PubMed
  66. Brouwer M et al. 2019. EMBO J. 38(17):e101289 . PubMed
  67. Ben Haim L, et al. 2021. Glia. 69:2812. PubMed
  68. Olguin SL, et al. 2022. Commun Biol. 5:672. PubMed
  69. Chludzinski E, et al. 2022. Front Vet Sci. 8:795126. PubMed
  70. Dang T, et al. 2017. Mol Psychiatry. 10.1038/mp.2016.253. PubMed
  71. Zunke F et al. 2017. Neuron. 97(1):92-107 . PubMed
  72. Chierto E, et al. 2018. Mol Neurobiol. 56:4231. PubMed
  73. OIsen T, et al. 2021. Front Cell Neurosci. 15:721371. PubMed
  74. Feng T, et al. 2021. J Cereb Blood Flow Metab. 41:1437. PubMed
  75. Novakova SS, et al. 2020. Tissue Eng Part A. 26:837. PubMed
  76. Michaels NJ, et al. 2020. J Neurosci. 8587:40. PubMed
  77. Pan X, et al. 2021. eLife. 10:00. PubMed
  78. Kahriman A, et al. 2021. Acta Neuropathologica Communications. 9(1):118. PubMed
  79. Kugler C, et al. 2020. Cell Rep Med. 1:100159. PubMed
  80. Li H, et al. 2016. Sci Rep. 6:29878. PubMed
  81. Dominguez N et al. 2017. Journal of neurochemistry. 144(3):241-254 . PubMed
  82. Wegmann S, et al. 2021. Sci Adv. 7:. PubMed
  83. Xie X, et al. 2021. Nat Commun. 12:4113. PubMed
  84. Rodriguez BL, et al. 2020. PLoS One. 15:e0239152. PubMed
  85. Hines TJ et al. 2017. eNeuro. 5(1) pii: ENEURO. PubMed
  86. Ban Y, et al. 2021. Sci Adv. 7:eabh2974. PubMed
  87. Lee CH, et al. 2020. Nat Commun. 4.466666667. PubMed
  88. Connor M, et al. 2021. PLoS One. 16:e0253766. PubMed
  89. Chueh KS, et al. 2022. Int J Mol Sci. 23:. PubMed
  90. Qian X, et al. 2020. Cell Stem Cell. 26(5):766-781. PubMed
  91. Mendsaikhan A, et al. 2019. Front Mol Neurosci. 11:470. PubMed
  92. Davies AK, et al. 2022. Nat Commun. 13:1058. PubMed
  93. Sellgren C, et al. 2016. Mol Psychiatry. 22:170-177. PubMed
  94. Domise M, et al. 2019. Cell Death Dis. 10:221. PubMed
  95. Olmsted ZT, et al. 2021. iScience. 24(8):102827. PubMed
  96. Wegscheid ML, et al. 2021. Cell Reports. 36(1):109315. PubMed
  97. Xiaojie Wang, Colin Studholme 2017. J Neurosci. 37(8):1971-1983. PubMed
  98. Li T, et al. 2016. Nat Commun. 7:12082. PubMed
  99. Wischhof L, et al. 2018. Mol Metab. 14:e1007363. PubMed
  100. Cousin MA, et al. 2021. Nat Genet. 53:1006. PubMed
RRID
AB_2566782 (BioLegend Cat. No. 837904)

Antigen Details

Structure
Three major neurofilament subunits. Its names given to these subunits are based upon the apparent molecular mass of the mammalian subunits on SDS-PAGE: The medium or middle (NF-M) runs at about 145-160 kD and the heavy or highest (NF-H) runs at 200-220 kD.
Distribution

Tissue distribution: CNS, peripheral nerves, and glandular cells of the prostate.
Cellular distribution: Cytoskeleton, nucleus, cytosol, and mitochondrion.

Function
Neurofilaments are the major components of the neuronal cytoskeleton. They provide axonal support and regulate axon diameter.
Cell Type
Mature Neurons
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Petzold A. 2005. J. Neurol. Sci. 233:183. PubMed

Gene ID
4747 View all products for this Gene ID 4744 View all products for this Gene ID 4741 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Neurofilament Marker
Specificity Alt (DOES NOT SHOW ON TDS):
Neurofilament Marker
App Abbreviation (DOES NOT SHOW ON TDS):
IHC-P,WB,EM,ICC,IHC-F
UniProt
View information about Neurofilament Marker on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 08/29/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account