Loading...

Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P)

Pricing & Availability
Clone
Poly18020 (See other available formats)
Regulatory Status
RUO
Other Names
CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, TUBB3, tubulin beta-4 chain, class III beta-tubulin
Previously
Covance Catalog# PRB-435P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
publications
A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918
IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • B_Poly18020_PURE_Tubulin_beta3_IHCP_2_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_Poly18020_PURE_Tubulin_beta3_IHCP_3_032918
    IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly18020_PURE_Tubulin_beta3_WB_021915.jpg
    Western blot of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.1 µg/mL of Poly18020 or Rabbit IgG overnight at 4°C, followed by incubation with HRP-labeled anti-rabbit IgG (Cat. No. 410406). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Save
802001 100 µL ¥95,260
Description

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against microtubules derived from rat brain.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 0.5 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunocytochemistry (ICC).

This antibody is well characterized and highly reactive to neuron specific Class III ß-Tubulin (ßIII), but does not identify ß-tubulin found in glial cells. This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Feuer R, et al. 2005. J. Neurosci. 25(9):2434-44.
  2. Mozzetti S, et al. 2005. Clin. Cancer Res. 11(1):298-305.
  3. Feuer R, et al. 2003. Am. J. Pathol. 163(4):1379-93.
  4. Zhang XM, Yang XJ. 2001. Development. 128(6):943-57.
  5. Ma M, et al. 2012. Neurobiol Dis. 56:34. (WB) PubMed
  6. Tseung G, et al. 2011. J. Virol. 85:5718. (IF) PubMed
  7. Shimada IS, et al. 2012. J Neurosci. 32:7926. (IHC) PubMed
  8. Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
Product Citations
  1. Tseng K, et al. 2017. eNeuro. 10.1523/ENEURO.0214-17.2017. PubMed
  2. Chow A, Morshead C 2016. Stem Cell Res. 16:79-87. PubMed
  3. Son M, et al. 2016. Exp Mol Med. 48: 232. PubMed
  4. Hoshino N, et al. 2021. Journal of Comparative Neurology. 529:3633. PubMed
  5. Ahangari N, et al. 2021. J Neuropathol Exp Neurol. 80:944. PubMed
  6. Samarasinghe RA, et al. 2021. Nat Neurosci. 24:1488. PubMed
  7. Yates PL, et al. 2021. Cell Mol Life Sci. 78:6941. PubMed
  8. Hodgetts SI, et al. 2022. Neural Regen Res. 17:1376. PubMed
  9. Muralidharan H, et al. 2022. J Neurosci. 42:2149. PubMed
  10. Jabali A, et al. 2022. EMBO Rep. 23:e54027. PubMed
  11. Reis LTC, et al. 2022. Environ Toxicol. 37:2044. PubMed
  12. Rodriguez CM, et al. 2022. Cell Rep. 41:111505. PubMed
  13. Matsubayashi J, et al. 2023. J Neurochem. 164:29. PubMed
  14. Libanori A, et al. 2023. Adv Mater. 35:e2206933. PubMed
  15. Shimizu K, et al. 2022. Cells. 11: . PubMed
  16. Olmsted ZT, et al. 2022. STAR Protoc. 3:101898. PubMed
  17. Mogas Barcons A, et al. 2022. Materials (Basel). 16: . PubMed
  18. Yates PL, et al. 2023. Front Cell Neurosci. 16:979652. PubMed
  19. Genchi A, et al. 2023. Nat Med. 29:75. PubMed
  20. Chiu YJ, et al. 2023. Biomol Ther (Seoul). :. PubMed
  21. Willumsen N, et al. 2023. Brain Commun. 5:fcac321. PubMed
  22. Yang OJ, et al. 2023. Front Neurosci. 17:1001007. PubMed
  23. Stojkovska I, et al. 2022. Neuron. 110:436. PubMed
  24. Prieto Huarcaya S, et al. 2022. Autophagy. 18:1127. PubMed
  25. Traxler L, et al. 2022. Cell Metab. 34:1248. PubMed
  26. Asamitsu S, et al. 2023. Sci Adv. 9:eade2035. PubMed
  27. Weng ZK, et al. 2023. Biomolecules. 13:. PubMed
  28. Tian JL, et al. 2023. Cells. 12:. PubMed
  29. Drobny S, et al. 2023. Transl Neurodegener. 12:31. PubMed
  30. Eltahir MK, et al. 2022. PLoS One. 17:e0269683. PubMed
  31. Pardieck J, et al. 2021. Stem Cells Dev. 30:816. PubMed
  32. Kim S, et al. 2021. Nat Commun. 12:1807. PubMed
  33. Kim JS, et al. 2020. Immunity. 54(1):176-190.e7. PubMed
  34. Tian E, et al. 2016. Cell Rep. 16: 781-792. PubMed
  35. Baleriola J, et al. 2016. Sci Rep. 6: 25928. PubMed
  36. Shimada I, et al. 2012. J Neurosci. 32:7926-7940. PubMed
  37. Arber C, et al. 2019. Brain Commun. 1:fcz024. PubMed
  38. Jiang Y, et al. 2018. J Biol Chem. 293:19317. PubMed
  39. Stokowska A, et al. 2017. Brain. 140:353. PubMed
  40. Brosig A, et al. 2019. Cell Rep. 29:2028. PubMed
  41. Girouard MP, et al. 2020. eneuro. 7(2):ENEURO. PubMed
  42. Krishnan V, et al. 2016. J Neuropathol Exp Neurol. 75: 464 - 478. PubMed
  43. Meng QT, et al. 2021. Elife. 10:. PubMed
  44. Olmsted ZT, et al. 2022. Front Cell Neurosci. 15:725195. PubMed
  45. Binder J, et al. 2022. Commun Biol. 5:125. PubMed
  46. Vogt M, et al. 2021. Theranostics. 11:5569. PubMed
  47. Pires G, et al. 2021. Brain Commun. 3:fcab021. PubMed
  48. Nichterwitz S, et al. 2020. Genome Res. 30:1083. PubMed
  49. Xie Y, et al. 2018. Stem Cell Res. 9:2010. PubMed
  50. Uezono N, et al. 2018. Stem Cells. 36:737. PubMed
  51. Tang SC, et al. 2017. Diabetologia. 61:158. PubMed
  52. MOSES C, et al. 2020. Sci Rep. 10:11393. PubMed
  53. Nayak G, et al. 2020. Cell Reports. 30(3):672-686.e8.. PubMed
  54. Brykczynska U, et al. 2016. Stem Cell Reports. 7:1059-1071. PubMed
  55. Omichi R, et al. 2020. Mol Ther Methods Clin Dev. 1.51875. PubMed
  56. Dvoriantchikova G, et al. 2022. Sci Rep. 12:17152. PubMed
  57. Jo S, et al. 2021. Stem Cell Research. 56:102524. PubMed
  58. Gong C, et al. 2021. Cell Reports. 34(12):108889. PubMed
  59. Kurihara Y, et al. 2020. Journal of Neurochemistry. 155(3):285-299. PubMed
  60. Hastings RL, et al. 2020. Sci Rep. 10:11132. PubMed
  61. Hoffman LF, et al. 2018. J Assoc Res Otolaryngol. 19:33. PubMed
  62. Carlin D et al. 2018. eNeuro. 5(2) pii: ENEURO. PubMed
  63. Sakaguchi H, et al. 2019. Stem Cell Reports. 13:458. PubMed
  64. Voronova A et al. 2017. Neuron. 94(3):500-516 . PubMed
  65. Sabelström H, et al. 2019. Cell Rep. 28:2064. PubMed
  66. Boland M, et al. 2017. Brain. 10.1093/brain/aww357. PubMed
  67. Ou MY, et al. 2021. Cell Reports. 35(13):109290. PubMed
  68. Mertens J, et al. 2021. Cell Stem Cell. 28:1533. PubMed
  69. van Niekerk EA, et al. 2022. Cell Rep Methods. 2:100255. PubMed
  70. Avraham O, et al. 2021. Elife. 10:. PubMed
  71. Simone R, et al. 2021. Nature. 594:117. PubMed
  72. Lasagni Vitar RM, et al. 2021. Invest Ophthalmol Vis Sci. 62:26. PubMed
  73. Reddy A, et al. 2020. Cell. 183:62. PubMed
  74. Otani T, et al. 2016. Cell Stem Cell. 18: 467-480. PubMed
  75. Verstraelen P, et al. 2017. Front Cell Neurosci. 11:173. PubMed
  76. Kim H et al. 2017. Stem cell research. 26:72-75 . PubMed
  77. Li H, et al. 2019. iScience. 19:559. PubMed
  78. Benoy V, et al. 2018. Brain. 10.1093/brain/awx375. PubMed
  79. Chabrat A, et al. 2019. Stem Cells Int. 2019:2945435. PubMed
  80. Yang C, et al. 2020. Neuron. 105:276. PubMed
  81. Suter TACS, et al. 2020. Cell Rep. 30:1164. PubMed
  82. Macpherson P, et al. 2015. Development. 142: 4038 - 4048. PubMed
  83. Ferguson M, et al. 2013. Neurobiol Dis. 56:34-46. PubMed
  84. Yau K, et al. 2016. J Neurosci. 36: 1071 - 1085. PubMed
  85. Cui Q, et al. 2016. Nat Commun. 7:10637. PubMed
  86. Bosze B, et al. 2020. J Neurosci. 40:1501. PubMed
  87. Adams MT, et al. 2021. eLife. 0.416666666666667. PubMed
  88. Iannielli A, et al. 2019. Cell Rep. 29:4646. PubMed
  89. Bowles KR, et al. 2019. PLoS One. 14:e0213374. PubMed
  90. Toda T, et al. 2017. Cell Stem Cell. 21:618. PubMed
  91. Qiang L et al. 2018. Current biology : CB. 28(13):2181-2189 . PubMed
  92. Meijer M et al. 2019. Cell Rep. 27(7):2199-2211 . PubMed
  93. Finno CJ, et al. 2022. Front Cell Neurosci. 16:853035. PubMed
  94. Newland B, et al. 2021. ACS Chem Neurosci. 12:1178. PubMed
  95. Amador A, et al. 2020. Brain. 143:2039. PubMed
  96. Sugiyama T, et al. 2021. iScience. 24(7):102758. PubMed
  97. Bhaskaran S, et al. 2020. Aging Cell. 19:e13225. PubMed
  98. Rhee HJ et al. 2019. Cell Rep. 27(7):2212-2228 . PubMed
  99. Makar T, et al. 2018. Stem Cell Res Ther. 9:127. PubMed
  100. Zahr SK et al. 2018. Neuron. 97(3):520-537 . PubMed
  101. Cora V, et al. 2019. Cells. 8:5. PubMed
  102. Grove M, et al. 2020. eLife. 9:e50138.. PubMed
  103. Francistiová L, et al. 2022. Front Mol Neurosci. 14:793769. PubMed
  104. Iennaco R, et al. 2022. Cell Death Differ. 29:293. PubMed
  105. Bjorke B, et al. 2021. Dev Biol. 476:272. PubMed
  106. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  107. Lin P, et al. 2016. Am J Physiol Gastrointest Liver Physiol. 311: G412 - G422. PubMed
  108. Tsueng G, et al. 2011. J Virol. 85:5718-5732. PubMed
  109. Alvarez–Lindo N, et al. 2019. Sci Rep. 9:18486. PubMed
  110. Tiwari D et al. 2019. Neurobiol Dis. 1871:48:00 . PubMed
  111. Carlin D et al. 2019. eNeuro. 6(3) pii: ENEURO. PubMed
  112. Li H, et al. 2018. Hum Mutat. 39:550. PubMed
  113. Ou J et al. 2018. Cell. 173(4):851-863 . PubMed
  114. Hsu J, et al. 2019. Nat Commun. 10:2939. PubMed
  115. Chanda S et al. 2019. Cell Stem Cell. 25(1):103-119 . PubMed
  116. Peitz M, et al. 2020. STAR Protoc. 1:100038. PubMed
  117. Watanabe S, et al. 2016. EMBO Mol Med. 8(12):1421-1437. PubMed
  118. Cates K, et al. 2020. Cell Stem Cell. 28(1):127-140.e9. PubMed
  119. Smith LA, et al. 2021. Front Cell Dev Biol. 9:667246. PubMed
  120. Mao Y, et al. 2021. Cell Prolif. 54:e13090. PubMed
  121. Olmsted ZT, et al. 2022. iScience. 25:104486. PubMed
  122. Vahsen BF, et al. 2022. Sci Rep. 12:12606. PubMed
  123. Flitsch LJ, et al. 2022. Sci Rep. 12:8356. PubMed
  124. Pahl MC, et al. 2021. Nat Commun. 12:6749. PubMed
  125. Kim H, et al. 2020. Sci Signal. 13: . PubMed
  126. Vicidomini C, et al. 2016. Mol Psychiatry. 10.1038/mp.2016.30. PubMed
  127. Barry DM, et al. 2020. Nat Commun. 11:1397. PubMed
  128. Hattori Y, et al. 2020. Nat Commun. 11:1631. PubMed
  129. Duncan DS, et al. 2018. Front Neurosci. 0.9875. PubMed
  130. Martínez JC et al. 2019. Neuron. 104(5):931-946 . PubMed
  131. Mickle AD, et al. 2019. Nature. 565:361. PubMed
  132. Kawakami Y et al. 2018. The Journal of Neuroscience. 38(10):2589-2604 . PubMed
  133. Shah K, et al. 2019. J Mol Cell Biol. N/A. PubMed
  134. Sar Shalom H, et al. 2019. Elife. 8:e41162. PubMed
  135. Facchin C, et al. 2020. Theranostics. 10:3518. PubMed
  136. Flores-Otero J, et al. 2007. J Neurosci. 27:14023-14034. PubMed
  137. Zhang L, et al. 2016. Neuroscience. 337:88-97. PubMed
  138. Arber C, et al. 2021. Cell Reports. 34(2):108615. PubMed
  139. Baker L, et al. 2021. JCI Insight. 6:. PubMed
  140. Besusso D, et al. 2020. Stem Cell Res. 102016:49. PubMed
  141. Shang Y, et al. 2018. J Neurosci. 38:4006. PubMed
  142. Jean-Louis T, et al. 2018. Neurobiol Aging. 62:130. PubMed
  143. Hung COY et al. 2018. Cell reports. 25(13):3647-3660 . PubMed
  144. Jeon H, et al. 2018. J Clin Med. 7:. PubMed
  145. Arber C, et al. 2020. Mol Psychiatry. 25:2919. PubMed
  146. Smith-Anttila CJA, et al. 2021. Developmental Biology. 476:18-32. PubMed
  147. Hung C, et al. 2021. Cell Reports. 35(11):109259. PubMed
  148. Ornaghi F, et al. 2020. Neurobiol Dis. 134:104667. PubMed
  149. Dan W, et al. 2022. Front Physiol. 12:744294. PubMed
  150. Avraham O, et al. 2022. Front Mol Neurosci. 14:796070. PubMed
  151. Jacob L, et al. 2022. J Exp Med. 219:. PubMed
  152. Avraham O, et al. 2022. Front Mol Neurosci. 15:967472. PubMed
  153. Su Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  154. Olmsted ZT, et al. 2021. iScience. 24(8):102827. PubMed
  155. Ward AI, et al. 2020. MBio. 11:00. PubMed
  156. Avraham O, et al. 2020. Nat Commun. 3.854861111. PubMed
  157. Anastasiadou S, Knöll B 2016. Exp Neurol. 279:243-260. PubMed
  158. Lee J, et al. 2018. J Clin Med. 7:. PubMed
RRID
AB_2564645 (BioLegend Cat. No. 802001)

Antigen Details

Cell Type
Mature Neurons, Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Microtubules
Gene ID
10381 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Tubulin beta-3
Specificity Alt (DOES NOT SHOW ON TDS):
Tubulin β-3 (TUBB3)
App Abbreviation (DOES NOT SHOW ON TDS):
IHC-P,WB,ICC
UniProt
View information about Tubulin beta-3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10/24/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account