- Clone
- TW4-2F8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Latency Associated Peptide (LAP), Transforming growth factor beta 1 (TGF-b1), TGFB1, DPD1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Human peripheral blood mononuclear cells were stimulated with CD3, CD28, and recombinant human IL-2 for 24-hours; cells were surface stained with LAP (clone TW4-2F8) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom), followed by intracelluar staining with FOXP3 PE (gated on CD4+ lymphocytes). -
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TGF-β1 (transforming growth factor β1) is a multifunctional cytokine which regulates cellular proliferation and differentiation. It is ubiquitously expressed by many types of cells. Platelets express high level of TGF-β. TGF-β is synthesized as a large protein precursor and then secreted as a complex of TGF-β and LAP (latency-associated peptide), in which LAP noncovalently associates with the dimeric mature TGF-β to prevent its activity. TGF-β requires activation before it binds to its receptors and exerts functions. It has been reported that LAP-TGF-β binds to the integrins αvβ1, αvβ6, αvβ8, and α8β1 through RGD domain. TGF-β plays important roles in control proliferation and differentiation of epithelial cells, endothelial cells, fibroblasts, neurons, osteoclasts, and osteoblasts. TGF-β is believed to be important in the regulation of the development of Treg, Th17, and Th9 cells. A recent study has shown that LAP is an activated Treg surface marker.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- LAP (TGF-β)
- Application
-
FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
Antigen Details
- Structure
- About 400 amino acids, N-terminal signal peptide is required for secretion from a cell, a pro-region (LAP) and C-terminal region that becomes the mature TGF-β molecule following its release from the pro-region by proteolytic cleavage.
- Distribution
-
Ubiquitously expressed by many kinds of cells
- Function
- Multifunctional cytokine, regulates cells proliferation, and differentiation
- Ligand/Receptor
- TGF-βRI, -RII, -RIII, CD105, LTBP, integrins
- Cell Type
- B cells, Dendritic cells, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroinflammation, Neuroscience, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Yi JJ, et al. 2010. Cell 142:144.
2. Tran DQ, et al. 2009. Blood 113:5125.
3. Lu M, et al. 2002. J. Cell. Sci. 115:4641.
4. Khalil N. 1999. Microbes Infect. 1:1255. - Gene ID
- 7040 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- LAP
- Specificity Alt (DOES NOT SHOW ON TDS):
- LAP
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about LAP on UniProt.org
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