- Clone
- Poly19252 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Prospero Homeobox Protein 1, Prox-1, Prospero-Related Homeobox 1, Homeobox Prospero-Like Protein Prox1
- Previously
-
Covance Catalog# PRB-238C
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Prox1 (Poly19252) detection on mouse brain. Photo courtesy of C. Zhou & S. Pleasure, UCSF. -
Prox1 (Poly19252) detection on mouse brain. Photo courtesy of S. Pleasure, UCSF. -
Total lysates (15 µg protein) from HepG2 (Positive control) and HL-60 (Low expression negative control) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 purified Prox1 antibody, clone Poly19052. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
| Cat # | Size | Price | Save |
|---|---|---|---|
| 925202 | 25 µL | ¥19,580 | |
| 925201 | 200 µL | ¥77,440 |
Prox1 is a prospero related homeobox gene. Its expression pattern suggests it has a role in a variety of embryonic tissues. Prox1-deficient mice are devoid of lymphatic vasculature, and in these animals endothelial cells fail to acquire the lymphatic phenotype; instead, they remain as blood vascular endothelium.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- The Prox1 antiserum was generated against the C-terminal 15 amino acids of mouse Prox1.
- Formulation
- Stored in 50% Glycerol and 0.03% Thimerosal.
- Preparation
- Serum
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
IHC-F - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a dilution of 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1:500 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).
*Expected MW: 83 kD
WB Positive Control: HEK293 Lysate
IHC Positive Control: Mouse Brain
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Pierreux CE, et al. 2004. J. Biol. Chem. 279:51298.
- Bagri A, et al. 2003. Cell. 113:285.
- Bagri A, et al. 2002. Development. 129:4249.
- Product Citations
-
- RRID
-
AB_2749865 (BioLegend Cat. No. 925202)
AB_2749865 (BioLegend Cat. No. 925201)
Antigen Details
- Cell Type
- Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells
- Gene ID
- 5629 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Prox1
- Specificity Alt (DOES NOT SHOW ON TDS):
- Prox1
- App Abbreviation (DOES NOT SHOW ON TDS):
- IHC-F,WB,ICC
- UniProt
- View information about Prox1 on UniProt.org
Related FAQs
Other Formats
View All Prox1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Anti-Prox1 | Poly19252 | IHC-F,WB,ICC |
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