- Clone
- Poly5313 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin-1α, Lymphocyte-activating factor (LAF), Endogenous pyrogen (EP), Leukocyte endogenous mediator (LEM), Mononuclear cell factor (MCF/MNCF)(il-1a,il1a)
- Isotype
- Goat Polyclonal Ig
- Ave. Rating
- Submit a Review
- Product Citations
- publications
IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1α is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses. Recombinant human IL-1α is an 18.0 kD protein containing 159 amino acids.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Immunogen
- Recombinant human IL-1α
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
- Application
-
ELISA Detection - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.25 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-1α recombinant protein ranging from 200 to 1.56 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance.
- Application Notes
-
The biotinylated Poly5313 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified 343-3B3-14 (Cat. No. 500104) antibody as the capture antibody.
Note: For human IL-1a in cell culture supernatant, serum, plasma or other bodily fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 434907 & 434908) are specially developed and recommended.
- RRID
-
AB_2686960 (BioLegend Cat. No. 531304)
Antigen Details
- Structure
- Cytokine
- Distribution
-
Monocytes, tissue macrophages, Langerhans cells, dendritic cells, T and B cells, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglias, gliomas, keratinocyte
- Function
- Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
- Interaction
- B cells, T cells, monocytes, dendritic cells
- Ligand/Receptor
- Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
- Cell Type
- Astrocytes, B cells, Dendritic cells, Endothelial cells, Fibroblasts, Langerhans cells, Macrophages, Monocytes, NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Antigen References
-
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C et al. 1996. Blood 87:2095. - Gene ID
- 3552 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- IL-1alpha
- Specificity Alt (DOES NOT SHOW ON TDS):
- IL-1α
- App Abbreviation (DOES NOT SHOW ON TDS):
- ELISA Detection
- UniProt
- View information about IL-1alpha on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Other Formats
View All IL-1α Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-human IL-1α | Poly5313 | ELISA Detection |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Biotin anti-human IL-1α
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