- Regulatory Status
- RUO
- Other Names
- IL-10 ELISA Kit
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Mouse IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) due to its ability to inhibit cytokine production by TH1 clones. IL-10 is expressed in activated CD8+ and CD4+ T cells, activated monocytes, mast cells, and Ly-1 B cells. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The functions of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production.
ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation.
BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.
Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.
Kit Contents
- Kit Contents
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- Mouse IL-10 ELISA MAX™ Capture Antibody (200X)
- Mouse IL-10 ELISA MAX™ Detection Antibody (200X)
- Mouse IL-10 Standard
- Avidin-HRP (1000X)
- Substrate Solution F
- Coating Buffer B (5X)
- Assay Diluent A (5X)
Older lots of these products may contain Substrate Solutions A and B instead of Substrate Solution F mentioned on the TDS and the current manual. Please refer to the lot-specific CoA for the list of the reagents included in the lot and follow the ELISA procedure provided there.
Product Details
- Verified Reactivity
- Mouse
- Application
-
ELISA
- As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
-
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- Sensitivity
- 16 pg/mL
- Standard Range
- 31.3-2,000 pg/mL
- Materials Not Included
-
- Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
- Deionized (DI) water
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
- Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
- Wash bottle or automated microplate washer
- Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
- Plate Sealer
- Absorbent paper
Antigen Details
- Cell Sources
- Activated CD8+ T cells, Th0, Th2 subset of CD4+ T cells, Ly-1+ B cells, monocytes, macrophages, keratinocytes
- Cell Type
- Tregs
- Biology Area
- Immunology
- Molecular Family
- Cytokines/Chemokines
- Gene ID
- 16153 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- IL-10
- Specificity Alt (DOES NOT SHOW ON TDS):
- IL-10
- App Abbreviation (DOES NOT SHOW ON TDS):
- ELISA
- UniProt
- View information about IL-10 on UniProt.org
Related FAQs
- Do the ELISA MAX™ Deluxe Sets come with plates?
-
No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.
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