PE/Dazzle 594 anti-mouse CD25 Antibody anti-CD25

Clone: 3C7 (See other available formats)
Regulatory Status: RUO
Other Names: IL-2Rα, Ly-43, p55, Tac
Isotype: Rat IgG2b, κ
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Product Citations: publications

3C7_PEDZL_CD25_Antibody_121417 — ConA-stimulated BALB/c splenocytes (Day 3) stained with CD25 (Clone 3C7) PE/Dazzle™ 594 (filled histogram) or rat IgG2b, κ PE/Dazzle™ 594 isotype control (open histogram).

Compare all formats See PE/Dazzle™ 594 spectral data

Cat #	Size	Price	Save
101919	25 µg	¥25,960
101920	100 µg	¥68,420

Description

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors^1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).
RRID: AB_2721701 (BioLegend Cat. No. 101919)
AB_2721701 (BioLegend Cat. No. 101920)

Antigen Details

Structure: Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution: Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells
Function: IL-2 receptor, signal transduction
Ligand/Receptor: IL-2
Cell Type: B cells, T cells, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.
Gene ID: 16184 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):: CD25
Specificity Alt (DOES NOT SHOW ON TDS):: CD25
App Abbreviation (DOES NOT SHOW ON TDS):: FC
UniProt: View information about CD25 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Other Formats

View All CD25 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse CD25	3C7	FC
Purified anti-mouse CD25	3C7	FC,CyTOF®,IHC-F,Block
FITC anti-mouse CD25	3C7	FC
APC anti-mouse CD25	3C7	FC
PerCP/Cyanine5.5 anti-mouse CD25	3C7	FC
Purified anti-mouse CD25 (Maxpar® Ready)	3C7	FC,CyTOF®
PE/Cyanine7 anti-mouse CD25	3C7	FC
APC/Cyanine7 anti-mouse CD25	3C7	FC
PE/Dazzle™ 594 anti-mouse CD25	3C7	FC
APC/Fire™ 750 anti-mouse CD25	3C7	FC
Brilliant Violet 421™ anti-mouse CD25	3C7	FC
Ultra-LEAF™ Purified anti-mouse CD25	3C7	FC,CyTOF®,IHC-F,Block

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 12/14/2017

For Research Use Only. Not for diagnostic or therapeutic use.

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