- Clone
- Syn-F1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-amyloid beta component (NAP)
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IHC staining of α-Synuclein deposits with anti-α-Synuclein, aggregated antibody (clone Syn-F1) on formalin-fixed paraffin-embedded Parkinson's disease brain tissue. Following antigen retrieval using citrate and 0.25% Triton X-100, the tissue was incubated with the primary antibody at 1 µg/mL overnight at 4°C. Biolegend's Ultra-Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided. -
Dot-blot of anti-α-Synuclein antibodies (clones Syn-F1 and 4B12/Synuclein) and isotype-matched IgG1 control, demonstrating their binding to α-synuclein conformers (monomeric and fibrillar). Note that these antibodies do not cross-react with amyloid beta peptide (Aβ1-42). Blots were incubated at room temperature for two hours with 0.5 µg/mL of the primary antibodies, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody (clone Poly4053). Enha
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α-Synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are α-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are a major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Aggregated α-synuclein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
Dot-Blot - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For Dot Blot, a concentration range of 0.5 - 3.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone does not cross-react with ß- or ?-synucleins using Dot Blot as detection method.
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Application References
(PubMed link indicates BioLegend citation) -
- Vaikath NN, et al. 2015. Neurobiol Dis. 79:81. (IHC, ELISA Capture, Dot-Blot)
Antigen Details
- Structure
- α-synuclein's canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
- Distribution
-
Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.
- Cell Sources
- Primarily nervous system, but lower expression in other tissues.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- α-Synuclein
- Antigen References
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1. Mor DE, et al. 2016. Neurobiol Dis. 88:66.
2. Jucker M, Walker LC. 2013. Nature. 501(7465):45.
3. Bartels T, et al. 2011. Nature. 477(7362):107.
4. Devine MJ, et al. 2011. Mov Disord. 26:2160. - Gene ID
- 6622 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- alpha-Synuclein, aggregated
- Specificity Alt (DOES NOT SHOW ON TDS):
- α-Synuclein, aggregated
- App Abbreviation (DOES NOT SHOW ON TDS):
- IHC-P, Dot Blot
- UniProt
- View information about alpha-Synuclein, aggregated on UniProt.org
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