- Clone
- O95C1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Tubulin 5 β, Tubulin β-4 chain
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Whole cell extracts (15 µg total protein) prepared from HeLa (Lane 1), Jurkat (Lane 2), NTERA-2 (lane 3), and NIH/3T3 (Lane 4) cells were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 0.1 µg/mL (1:5000 dilution) purified anti-β-Tubulin antibody (Clone O95C1). Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG (Cat. No. 405301). Equal protein loading was confirmed using Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) at a 1:5000 dilution (lower). Lane M: MW ladder.
| Cat # | Size | Price | Save |
|---|---|---|---|
| 605101 | 25 µg | ¥22,220 | |
| 605102 | 100 µg | ¥55,660 |
Eukaryotic microtubules are tubular polymers of tubulin that are a major component of the cytoskeleton. They play an essential role in diverse cellular functions, including migration, mitosis, and trafficking, and are also critical for normal development. β- and α-tubulins comprise the two core protein families that heterodimerize and assemble into each microtubule filament. There are multiple β- and α-tubulins isotypes, with each showing unique tumoral and tissue-specific distribution. TUBB4A is a β–tubulin primarily expressed in nervous tissue and moderate expression in testis. Mutations in TUBB4A cause hypermyelinating leukodystrophy-6 and autosomal dominant torsion dystonia -4.
Product DetailsProduct Details
- Verified Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length protein expressed in HEK293T cells transfected with human TUBB4 expression vector.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (1:500-1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Because of complete sequence homology, this clone is also predicted to recognize TUBB4A in higher eukaryotes, including rat, monkey, pig, bovine, and canine. This clone was generated using full-length recombinant TUBB4A as an immunogen. However, due to high sequence homology, this clone may also recognize other members of the ß-Tubulin family, notably TUBB2A, TUBB3, TUBB4B, and TUBB8. Because these ß-Tubulins have a similar molecular weight as TUBB4A, we do not recommend using this antibody as a TUBB4A-specific antibody. This clone was generated using a more defined immunogen (recombinant TUBB4A) than our existing ß-Tubulin clone, TU27/Tubulin (Cat. No. 903401), which was generated against taxol-assembled microtubules (mixed tubulins).
- Product Citations
-
- RRID
-
AB_2728462 (BioLegend Cat. No. 605101)
AB_2728462 (BioLegend Cat. No. 605102)
Antigen Details
- Structure
- TUBB4A is a 444 amino acid protein with a predicted molecular weight of 49.58 kD.
- Distribution
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β-Tubulins are ubiquitously expressed, with TUBB4A showing highest expression levels in brain and moderate expression in testis. TUBB4A shows low expression in other tissues.
- Function
- Cell migration, cell trafficking, mitosis, cilia and flagella motor dynamics.
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Microtubules
- Antigen References
-
- Arai-Ichinoi N, et al. 2016. Hum. Genet. 135: 89.
- Romaniello R, et al. 2015. Brain Dev. 37: 273.
- Shimojima K, et al. 2015. Brain Dev. 37: 281.
- Pizzino A, et al. 2014. Neurology. 83: 898.
- Tamura D, et al. 2013. Cancer Med. 2: 144.
- Hersheson J, et al. 2013. Ann. Neurol. 73: 546.
- Gene ID
- 10382 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- beta-Tubulin
- Specificity Alt (DOES NOT SHOW ON TDS):
- β-Tubulin
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB
- UniProt
- View information about beta-Tubulin on UniProt.org
Related FAQs
Other Formats
View All β-Tubulin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-β-Tubulin | O95C1 | WB |
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Purified anti-β-Tubulin
Whole cell extracts (15 µg total protein) prepared from HeLa...
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