- Clone
- W19138A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Nitric oxide synthase (NOS1), neuronal NOS (N-NOS)
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Whole cell extracts (15 µg total protein) from the indicated tissue lysate or cell line were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 0.5 µg/mL (1:1000 dilution) of purified anti-NOS1 (clone W19138A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:25000 dilution (lower). Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent Lane M: Molecular weight marker -
IHC staining using purified anti-NOS1 (clone W19138A) on formalin-fixed paraffin-embedded human cerebellum tissue at 10x (left) and 40x (right) objectives. The white box indicates the region of the higher magnification image. The tissue was incubated with 10 μg/mL of the anti-NOS1 antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 Goat anti-rat IgG (red) (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. Scale bar = 50 μM
| Cat # | Size | Price | Save |
|---|---|---|---|
| 602851 | 25 µg | ¥25,960 | |
| 602852 | 100 µg | ¥64,460 |
Nitric oxide synthase 1 (NOS1) catalyzes the formation of nitric oxide (NO) from L-arginine. NO signals in neurons intracellularly to regulate metabolic activity and dendrite growth. NO also functions in neurons as a neurotransmitter-like signaling molecule, and in muscle cells, where it is a potent relaxer. High concentrations of NO are cytotoxic, so it is utilized by macrophages as a bactericide/tumoricide, and it has also been implicated in the progression of neurodegenerative disorders like Alzheimer’s.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant fragment of human NOS1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product was tested and failed for IHC-F in mouse brain.
- RRID
-
AB_2910479 (BioLegend Cat. No. 602851)
AB_2910479 (BioLegend Cat. No. 602852)
Antigen Details
- Structure
- NOS1 is a 1,434 amino acid protein with a predicted molecular weight of 161 kD.
- Distribution
-
NOS1 is expressed in the cytoplasm of neurons, skeletal and smooth muscle cells, and macrophages.
- Function
- NOS1 is an enzyme that synthesizes nitric oxide.
- Cell Type
- Macrophages, Mature Neurons, Neurons
- Antigen References
-
- Kourosh-Arami M, et al. 2019. Rev. Neurosci. 31:617-636.
- Jin R and Loscalzo J. 2010. J. Blood Med. 1:147-162.
- Gene ID
- 4842 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- NOS1
- Specificity Alt (DOES NOT SHOW ON TDS):
- NOS1
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,IHC-P
- UniProt
- View information about NOS1 on UniProt.org
Related FAQs
Other Formats
View All NOS1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-NOS1 | W19138A | WB,IHC-P |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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