- Clone
- S100A4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Protein S100-A4, calvasculin, protein 18A2, protein Mts1, metastatic cell protein, S100 calcium-binding protein A4, placental calcium-binding protein, leukemia multidrug resistance associated protein, 18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98
- Previously
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Covance Catalog# PRB-497P
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Staining of PRB-497P on formalin-fixed paraffin embedded human tonsil.
The S100A4 protein belongs to the S100 subfamily, which has grown to be one of the largest subfamilies of the EF-hand Ca2+-binding proteins. The S100 proteins are small (10-12 kD), acidic proteins sharing 25-65% sequence homology. A majority of the S100 genes cluster on human chromosome 1q21. The discovery of this cluster has provided the basis for a new nomenclature for S100 proteins encoded by genes in the 1q21 region. These proteins are now designated S100A1-S100A13. In immunoblotting analysis using recombinant S100 proteins (S100A1, S100A2, S100A4, S100A6 and S100B) only the corresponding antigen (S100A4) was recognized. No cross-reaction with the other S100 proteins was observed.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Reported Reactivity
- Cat, Cow, Dog
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This antibody was raised against purified recombinant human S100A4 monomer expressed by E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.1 M NaCl, 0.015 M sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- Store between 2 - 8°C
- Application
-
IHC-P, ICC
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC-P: 1:100 - 1:200 for Biotin based detection systems such as USA Ultra Streptavidin Detection (929501).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Citrate Buffer Retrieval Solution (928502).
Incubation: 20 minutes at room temperature. - Application Notes
-
This antibody is effective in immunocytochemistry (ICC) and immunohistochemistry (IHC).
Predicted Molecular Weight = 10 - 12 kD
Postive Tissue: Tonsil (human)
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Schäfer BW, Heizmann CW. The S100 family of EF-hand calcium-binding proteins: functions and pathology. Trends Biochem Sci 21:134-40, 1996.
- Engelkamp D, Schäfer BW, Mattei MG, Erne P, Heizmann CV. Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E. Proc Natl Acad Sci 90:6547-51, 1993.
- Product Citations
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- RRID
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AB_2564748 (BioLegend Cat. No. 810101)
Antigen Details
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Immune Checkpoint Receptors
- Gene ID
- 20198 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- S100A4
- Specificity Alt (DOES NOT SHOW ON TDS):
- S100A4
- App Abbreviation (DOES NOT SHOW ON TDS):
- IHC-P,ICC
- UniProt
- View information about S100A4 on UniProt.org
Related FAQs
Other Formats
View All S100A4 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-S100A4 | S100A4 | IHC-P,ICC |
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Purified anti-S100A4
Staining of PRB-497P on formalin-fixed paraffin embedded hum...
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