Alexa Fluor 488 anti-Bcl-2 Antibody anti-Bcl-2

Clone: BCL/10C4 (See other available formats)
Regulatory Status: RUO
Other Names: B-cell leukemia 2
Isotype: Mouse IgG1, κ
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BCL10C4_A488_012210 — C57BL/6 splenocytes intracellular stained with BCL/10C4 Alexa Fluor® 488. Cells were fixed and permeabilized with FOXP3 Fix/Perm Buffer Set.

Compare all formats See Alexa Fluor® 488 spectral data

Cat #	Size	Price	Save
633505	25 µg	¥27,280
633506	100 µg	¥63,360

Description

Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-x_L, and Bag-1. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue⁴, immunocytochemical staining⁵, and immunoprecipitation⁵.
RRID: AB_2028387 (BioLegend Cat. No. 633505)
AB_2028387 (BioLegend Cat. No. 633506)

Antigen Details

Structure: Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
Distribution: Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
Function: Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Modification: Phosphorylation, Ubiquitination
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References: 1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214.
2. Yang E, et al. 1995. Cell 80:285.
3. Huang Z, et al. 2000. Oncogene 19:6627.
4. Deng X, et al. 2003. Blood. 102:3179.
Regulation: Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Gene ID: 596 View all products for this Gene ID
UniProt: View information about Bcl-2 on UniProt.org

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Other Formats

View All Bcl-2 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Bcl-2	BCL/10C4	WB,IP,ICC,IHC-F
FITC anti-Bcl-2	BCL/10C4	ICFC
Alexa Fluor® 488 anti-Bcl-2	BCL/10C4	ICFC
PE anti-Bcl-2	BCL/10C4	ICFC
Alexa Fluor® 647 anti-Bcl-2	BCL/10C4	ICFC
PE/Cyanine7 anti-Bcl-2	BCL/10C4	ICFC
Direct-Blot™ HRP anti-Bcl-2	BCL/10C4	WB

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Go To Top Version: 3 Revision Date: 09/11/2014

For Research Use Only. Not for diagnostic or therapeutic use.

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