Alexa Fluor 488 anti-Cyclin B1 Antibody anti-Cyclin B1

Clone: V152 (See other available formats)
Regulatory Status: RUO
Other Names: CCNB1
Isotype: Mouse IgG1
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V152_A488_CyclinB1_Antibody_1_101016 — Jurkat cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and Cyclin B1 (clone V152) Alexa Fluor® 488 (top) or mouse IgG1, κ Alexa Fluor® 488 isotype control (bottom).

Compare all formats See Alexa Fluor® 488 spectral data

Cat #	Size	Price	Save
647907	25 tests	¥32,340
647908	100 tests	¥71,060

Description

Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48 kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition. The V152 monoclonal antibody recognizes human and mouse Cyclin B1 and has been shown to be useful for Western blotting and immunohistochemistry (paraffin embedded tissue sections) of human and mouse cyclin B1.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Reported Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Hamster Cyclin B1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications include: immunohistochemistry² of formalin-fixed paraffin-embedded sections and immunofluorescence¹ of methanol fixed cells grown on glass coverslips.
RRID: AB_2632639 (BioLegend Cat. No. 647907)
AB_2632639 (BioLegend Cat. No. 647908)

Antigen Details

Structure: Belongs to the cyclin family. Cyclin AB subfamily.
Distribution: Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.
Interaction: CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4
Bioactivity: Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: 1. Morgan DO, et al.1995. Nature 374:131
Gene ID: 891 View all products for this Gene ID
UniProt: View information about Cyclin B1 on UniProt.org

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View All Cyclin B1 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Cyclin B1	V152	WB,ICC,IHC
PE anti-Cyclin B1	V152	ICFC
Alexa Fluor® 647 anti-Cyclin B1	V152	ICFC
Alexa Fluor® 488 anti-Cyclin B1	V152	ICFC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 10/10/2016

For Research Use Only. Not for diagnostic or therapeutic use.

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