IHC staining of Alexa Fluor® 488 anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of Alexa Fluor® 488 anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of Alexa Fluor® 488 anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of Alexa Fluor® 488 anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of Alexa Fluor® 488 anti-HNRNPA1 antibody (clone 4B10/HNRNPA1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a multi-purpose RNA binding protein involved in RNA metabolism and DNA genomic stability in normal and pathological conditions. hnRNP A1 is ubiquitously expressed and usually associates with nascent RNA polymerase II transcripts. hnRNP A1 is abundantly expressed in neuronal cells and reproductive tissue. Loss of hnRNP A1 has been observed in several neurodegenerative diseases, including Alzheimer’s disease, multiple sclerosis, amytrophic lateral sclerosis. Structurally, hnRNPA1 has two RNA recognition motifs, which are post-translationally modified to alter its nuclear pore shuttling properties and RNA interaction.
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
hnRNPA1 has two isoforms. Human HNRPNA1 isoform A1-A is a 320 amino acid protein with a molecular mass of 34 kD, and isoform A1-B is a 372 amino acid protein with a molecular mass of 38 kD.
Distribution
Tissue Distribution: Ubiquitous expression
Cellular Source: Nucleus and cytoplasm
Function
RNA metabolism, genomic stability
Interaction
Nascent RNA polymerase II transcripts
Cell Type
Neurons
Biology Area
Neurodegeneration, Neuroscience, Protein Synthesis
Antigen References
Bekenstein U, et al. 2013. Mol Cell Neurosci. 56:436-46.
Jean-Philippe J, et al. 2014. Biochim Biophys Acta. 1839(4): 251–258.
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