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Alexa Fluor® 488 anti-mouse CD31 Antibody

Pricing & Availability
Clone
MEC13.3 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
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Product Citations
publications
MEC13dot3_Alx488_080607
C57BL/6 mouse splenocytes stained with MEC13.3 Alexa Fluor® 488
  • MEC13dot3_Alx488_080607
    C57BL/6 mouse splenocytes stained with MEC13.3 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
Cat # Size Price Save
102513 25 µg ¥22,220
102514 100 µg ¥50,380
Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Polyoma middle T transformed EC line tEnd.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530).

Application References

(PubMed link indicates BioLegend citation)
  1. Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
  2. Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
  3. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  4. Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
  5. Baldwin HS, et al. 1994. Development 120:2539. (IHC)
  6. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  7. Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  10. Morita R, et al. 2015. PNAS. 112:160. PubMed
  11. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Tang X, et al. 2008. Neuroscience. 154:556. PubMed
  2. Rossi G, et al. 2014. J Cell Sci. 127:4788. PubMed
  3. Cheng Y, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:964. PubMed
  4. Smolen JA, et al. 2022. PNAS Nexus. 1:pgac235. PubMed
  5. Yu L, et al. 2022. Cancer Sci. 113:3980. PubMed
  6. Krausgruber T, et al. 2020. Nature. 583:296. PubMed
  7. Choudhury SR, et al. 2020. Cell. 178(5):1205-1221.e17.. PubMed
  8. Leung V, et al. 2009. Am J Pathol. 175:1757. PubMed
  9. Zhao G, et al. 2020. Sci Adv. 6:00. PubMed
  10. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
  11. Vandoorne K, et al. 2018. Circ Res. 123:415. PubMed
  12. Konno A, et al. 2020. Sci Rep. 10:12613. PubMed
  13. Wang C, et al. 2021. Cell Rep. 37:110021. PubMed
  14. Gui J, et al. 2020. Nat Cancer. 1:603. PubMed
  15. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  16. Gomez I, et al. 2020. Nat Commun. 11:214. PubMed
  17. Son NH, et al. 2018. J Clin Invest. 128:4329. PubMed
  18. Lu Q, et al. 2014. J Control Release. 174:43. PubMed
  19. Limbad C, et al. 2022. iScience. 25:103848. PubMed
  20. Clement CC, et al. 2021. Nat Commun. 12:4447. PubMed
  21. Fair-Mäkelä R, et al. 2020. Mucosal Immunol. 245:13. PubMed
  22. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  23. Wuebbles RD, et al. 2019. Mol Ther Methods Clin Dev. 13:145. PubMed
  24. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  25. Mana MD, et al. 2021. Cell Reports. 35(10):109212. PubMed
  26. Sakurai Y, et al. 2018. Mol Ther Oncolytics. 11:102. PubMed
  27. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  28. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
  29. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  30. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  31. Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed
  32. Takeda K, et al. 2014. Am J Pathol. 184:686. PubMed
  33. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  34. Kuo P, et al. 2016. J Am Heart Assoc. 5: 002610. PubMed
  35. Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed
  36. Crescente M, et al. 2020. FASEB J. 34:10027. PubMed
  37. Li Q, et al. 2020. Am J Pathol. 190:2453. PubMed
  38. Wegner S, et al. 2019. Mol Neurobiol. 57:1446. PubMed
RRID
AB_2161031 (BioLegend Cat. No. 102513)
AB_2161031 (BioLegend Cat. No. 102514)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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