- Clone
- RMG2a-62 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G2a
- Isotype
- Rat IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Save |
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407121 | 25 µg | ¥19,580 | |
407122 | 100 µg | ¥42,680 |
The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Blue Laser (488 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Gavin AL, et al.2006. Science 314:1936.
- Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
- Product Citations
-
- RRID
-
AB_2721368 (BioLegend Cat. No. 407121)
AB_2721368 (BioLegend Cat. No. 407122)
Related FAQs
- IgG2a gene is deleted in some mouse strains, which ones are they?
-
IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
Other Formats
View All IgG2a Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse IgG2a | RMG2a-62 | ELISA,FC |
Biotin anti-mouse IgG2a | RMG2a-62 | ELISA,FC |
FITC anti-mouse IgG2a | RMG2a-62 | FC |
PE anti-mouse IgG2a | RMG2a-62 | FC |
APC anti-mouse IgG2a | RMG2a-62 | FC |
PerCP/Cyanine5.5 anti-mouse IgG2a | RMG2a-62 | FC |
PE/Cyanine7 anti-mouse IgG2a | RMG2a-62 | FC |
Alexa Fluor® 647 anti-mouse IgG2a | RMG2a-62 | FC,IHC-P |
Brilliant Violet 421™ anti-mouse IgG2a | RMG2a-62 | FC |
Alexa Fluor® 488 anti-mouse IgG2a | RMG2a-62 | FC |
APC/Fire™ 750 anti-mouse IgG2a | RMG2a-62 | FC |
Alexa Fluor® 594 anti-mouse IgG2a | RMG2a-62 | IHC-F,IHC-P |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse IgG2a
-
Biotin anti-mouse IgG2a
-
FITC anti-mouse IgG2a
-
PE anti-mouse IgG2a
-
APC anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with (filled... -
PerCP/Cyanine5.5 anti-mouse IgG2a
Human peripheral blood lymphocyte were stained with purified... -
PE/Cyanine7 anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 647 anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with purifie... IHC staining of Alexa Fluor® 647 anti-mouse IgG2a on formali... -
Brilliant Violet 421™ anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 488 anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with purifie... -
APC/Fire™ 750 anti-mouse IgG2a
Human peripheral blood lymphocytes were stained with purifie... -
Alexa Fluor® 594 anti-mouse IgG2a
Human frozen tonsil section was fixed with 4% paraformaldehy... IHC staining of Alexa Fluor® 594 anti-mouse IgG2a on formali...
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