Alexa Fluor® 488 anti-RBFOX3 (NeuN) Antibody, RBFOX3, W18230A

Clone: W18230A (See other available formats)
Regulatory Status: RUO
Other Names: NeuN, RNA binding protein fox-1 homolog 3 (RBFox3), hexaribonucleotide binding protein 3, HRNBP3
Isotype: Rat IgG2a, κ
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W18230A_AF-488_RBFOX3_IHC-F_040924 — IHC staining of Alexa Fluor® 488 anti-RBFOX3 (NeuN) (clone W18230A) on frozen mouse brain tissue. The tissue was incubated without (panel A) and with (panel B) 5 µg/mL of Alexa Fluor® 488 anti- RBFOX3 (NeuN) (clone W18230A) overnight at 4°C. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective and merged. Scale bar: 50 µm

Compare all formats See Alexa Fluor® 488 spectral data

Cat #	Size	Price	Save
608455	25 µg	¥41,800
608456	100 µg	¥105,600

Description

RBFox3, commonly referred to as NeuN, is one of a family of 3 mammalian Fox homologues. Fox was discovered in C. elegans as a gene involved in sex determination, and the name RBFox is an acronym of "Feminizing locus on X". The Fox protein and its 3 mammalian homologues are all about 46 kD proteins, each of which includes a central highly conserved RRM type RNA recognition motif, which corresponds to a small ~70 amino acid structure consisting of 4 beta strands and two alpha-helices. RBFox3 is a protein which has a function in RNA splicing and is expressed heavily and specifically in neuronal nuclei. The antibody reveals strong nuclear and distal cytoplasmic staining for RBFox3/NeuN and the complete absence of staining of astrocytes and other kinds of non-neuronal cells. This RBFox3/NeuN antibody is therefore an excellent marker of neuronal cells.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Full length recombinant human RBFOX3, isoform 5
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-F - Quality tested
IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry on frozen tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunohistochemistry in paraffin-embedded tissues, a concentration range of 2.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Additional Product Notes: For immunohistochemistry in paraffin-embedded tissues (IHC-P), we recommend antigen retrieval with Sodium Citrate (Cat. No. 928502). Antigen retrieval with Tris-EDTA (10 mM Tris, 1 mM EDTA, 0.05% Tween-20, pH 9.0) results in dim signal.
RRID: AB_3106162 (BioLegend Cat. No. 608455)
AB_3106162 (BioLegend Cat. No. 608456)

Antigen Details

Structure: RBFox3 is a 312 amino acid protein with a predicted molecular weight of 34 kD
Distribution: Mature Neurons, Neural Stem Cells/ Cytoplasm and Nucleus
Function: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Cell Type: Mature Neurons, Neural Stem Cells
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Nuclear Markers
Antigen References: Dhekne HS, et al. 2018. Elife. 7:e40202.

Sun Y, et al. 2020. J Neuroinflammation. 17:72.

Schwabenland M, et al. 2021. Immunity. 54:1594-1610.
Gene ID: 146713 View all products for this Gene ID
UniProt: View information about RBFOX3 on UniProt.org

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View All RBFOX3 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-RBFOX3 (NeuN)	W18230A	WB,IHC-P,IHC-F,SB
Alexa Fluor® 647 anti-RBFOX3 (NeuN)	W18230A	IHC-P,IHC-F
Alexa Fluor® 488 anti-RBFOX3 (NeuN)	W18230A	IHC-F,IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 04/09/2024

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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