Alexa Fluor® 488 anti-Syntaxin Antibody, Syntaxin, SP8

Clone: SP8 (See other available formats)
Regulatory Status: RUO
Other Names: Syntaxin-1A, neuron-specific antigen HPC-1
Isotype: Mouse IgG1
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IHC staining of Alexa Fluor® 488 anti-Syntaxin antibody (clone SP8) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 200 µm

Compare all formats See Alexa Fluor® 488 spectral data

Cat #	Size	Price	Save
827009	25 µg	¥34,980
827010	100 µg	¥71,060

Description

Syntaxin, also known as syntaxin-1A and neuron-specific antigen HPC-1, is part of the Q-SNARE protein family that participates in exocytosis. Syntaxins have a SNARE domain, an N-terminal regulatory domain, and a single C-terminal transmembrane domain. Syntaxin drives the spontaneous calcium-independent fusion of synaptic vesicles that contain v-SNARE proteins, and may form part of the fusion pore of exocytosis.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: This monoclonal antibody was raised against a crude synaptic preparation from a post-mortem human brain.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes: This antibody is effective in immunohistochemistry (IHC-P) and western blotting (WB).
Additional Product Notes: The reactivity of this conjugate is weak in human tissue.
RRID: AB_2820131 (BioLegend Cat. No. 827009)
AB_2820131 (BioLegend Cat. No. 827010)

Antigen Details

Biology Area: Cell Biology, Neuroscience, Synaptic Biology
Molecular Family: Presynaptic proteins
Gene ID: 6804 View all products for this Gene ID
UniProt: View information about Syntaxin on UniProt.org

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Immunohistochemistry Protocol for Paraffin-Embedded Sections

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Other Formats

View All Syntaxin Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-Syntaxin	SP8	IHC-P,WB
HRP anti-Syntaxin	SP8	WB,IHC-P
Biotin anti-Syntaxin	SP8	WB,IHC-P
Alexa Fluor® 647 anti-Syntaxin	SP8	IHC-P
Alexa Fluor® 488 anti-Syntaxin	SP8	IHC-P

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12/12/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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