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Alexa Fluor® 594 Anti-Claudin 6 Antibody

Pricing & Availability
Clone
W22275B (See other available formats)
Regulatory Status
RUO
Other Names
Claudin-6, Claudin 6, CLDN6, Skullin
Isotype
Rat IgG2a, λ
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Product Citations
publications
W22275B_AF594_Claudin6_Antibody_1_022725
IHC staining with Alexa Fluor® 594 anti-Claudin 6 was performed on formalin-fixed paraffin-embedded human ovarian cancer tissue following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704). The tissue sections were incubated with Alexa Fluor® 594 Rat IgG2a, κ Isotype Control (Cat. No. 400555) (panel A) or with Alexa Fluor® 594 anti-Claudin 6 (clone W22275B) (panels B and C). Nuclei were counterstained with DAPI (Cat. No. 422801) (overlay in panels A and C). Scale Bar: 50 µm
  • W22275B_AF594_Claudin6_Antibody_1_022725
    IHC staining with Alexa Fluor® 594 anti-Claudin 6 was performed on formalin-fixed paraffin-embedded human ovarian cancer tissue following antigen retrieval using Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704). The tissue sections were incubated with Alexa Fluor® 594 Rat IgG2a, κ Isotype Control (Cat. No. 400555) (panel A) or with Alexa Fluor® 594 anti-Claudin 6 (clone W22275B) (panels B and C). Nuclei were counterstained with DAPI (Cat. No. 422801) (overlay in panels A and C). Scale Bar: 50 µm
See Alexa Fluor® 594 spectral data
Cat # Size Price Save
650053 25 µg ¥41,800
650054 100 µg ¥108,900
Description

Claudin-6 is a member of the claudin family of proteins, which consists of at least 24 members. Claudin proteins are integral membrane proteins characterized by 4 transmembrane regions and 2 extracellular loops that form tight junctions. Abnormal expression can disrupt tight junction integrity, playing an important role in the occurrence and development of cancers. Claudin-6 in particular, has been considered a therapeutic target because it is commonly expressed in tumor tissue but not in healthy adult tissue.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human Claudin-6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

For use of this antibody in immunohistochemistry on formalin-fixed paraffin embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704)

Antigen Details

Structure
Consists of four transmembrane domains and two extracellular loops. It is a 220 amino acid length protein with a predicted molecular weight of 23 kDa.
Distribution

Cellular membrane

Function
Tight junctions and cell adhesion
Antigen References
  1. Reinhard K, et al. 2020. Science. 367(6476):446-453.
  2. Huan D, et al. 2021. Mol Med Rep. 24(3):677.
  3. Ushiku T, et al. 2012. Histophatology. 61(6):1043-56.
  4. Gowrikumar S, et al. 2019. Int J Mol Sci. 21:53.
Gene ID
9074 View all products for this Gene ID
UniProt
View information about Claudin-6 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 02/27/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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